Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 245.2 3
0181-0448
 196.51 448.67 338 72.43 0.810
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 251.8 3
0182-0447
 315.10 447.79 367 74.91 0.822
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.1 3
0183-0452
 248.41 451.51 349 71.60 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.1 3
0184-0455
 527.89 453.68 409 81.84 0.767
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 205.8 3
0185-0461
 105.25 461.70 375 81.15 0.716
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 215.3 3
0186-0463
 71.14 463.23 353 73.26 0.827
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 218.5 3
0187-0465
 761.22 465.32 369 71.60 0.905
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 246.2 3
0188-0473
 624.44 473.31 375 72.43 0.898
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 206.4 3
0189-0476
 471.95 476.54 334 72.91 0.790
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 236.6 3
0190-0475
 851.66 474.90 353 71.01 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 181.5 3
0191-0476
 125.82 475.90 352 77.98 0.727
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 218.0 3
0192-0482
 929.08 482.82 380 78.33 0.778
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 237.2 3
0193-0481
 238.90 481.00 375 78.57 0.763
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.3 3
0194-0484
 565.52 484.47 368 73.26 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 240.2 3
0195-0488
 798.10 488.09 378 76.43 0.813
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 320.0 3
0196-0490
 720.05 490.02 371 72.67 0.883
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.2 3
0197-0495
 687.56 495.55 364 71.84 0.886
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 228.6 3
0198-0502
 601.36 502.21 361 73.01 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.5 3
0199-0504
 491.17 503.98 359 76.08 0.779
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.5 3
0200-0507
 230.55 507.90 370 78.33 0.758
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.0 3
0201-0504
 441.48 503.77 353 71.26 0.874
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 270.3 3
0202-0506
 356.38 506.34 353 72.43 0.846
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 234.7 3
0203-0506
 76.00 505.92 340 70.91 0.850
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.1 3
0204-0510
 806.46 509.69 392 81.40 0.743
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.2 3
0205-0523
 274.92 522.77 728 119.78 0.638