Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 271.3 1
0092-0294
 641.46 293.41 420 77.26 0.884
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 562.9 1
0093-0299
 840.45 299.21 344 68.18 0.930
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 294.7 1
0094-0300
 899.82 300.36 385 72.43 0.922
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 327.8 1
0095-0304
 436.31 304.50 925 116.23 0.860
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 304.4 1
0096-0303
 219.20 302.97 382 71.60 0.936
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 272.8 1
0097-0306
 109.51 306.11 396 77.26 0.834
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 566.9 1
0098-0310
 175.48 310.67 613 96.81 0.822
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 296.3 1
0099-0305
 37.12 304.96 376 74.08 0.861
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 349.8 1
0100-0309
 549.31 309.07 383 71.60 0.939
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 295.7 1
0101-0319
 681.97 319.46 401 75.01 0.896
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 260.5 1
0102-0319
 393.65 319.13 386 73.84 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.6 1
0103-0327
 934.12 327.39 370 71.26 0.916
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 297.1 1
0104-0333
 774.77 333.03 392 73.01 0.924
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.4 1
0105-0333
 582.47 333.28 913 135.05 0.629
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 368.6 1
0106-0336
 729.96 335.83 392 73.84 0.903
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 208.7 1
0107-0340
 823.92 339.51 324 70.33 0.823
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 236.4 1
0108-0344
 144.33 344.39 395 74.08 0.904
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 218.3 1
0109-0345
 180.49 344.57 405 77.50 0.847
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.4 1
0110-0347
 37.82 347.41 374 73.26 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 311.5 1
0111-0354
 298.60 353.98 743 108.13 0.799
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 260.9 1
0112-0351
 352.83 351.52 398 74.43 0.903
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 330.0 1
0113-0354
 106.35 354.35 382 73.84 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.4 1
0114-0357
 622.14 357.04 652 97.98 0.853
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 240.9 1
0115-0365
 201.37 366.55 896 121.98 0.757
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.6 1
0116-0370
 707.79 370.13 433 79.50 0.861