Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 265.2 3
0236-0596
 632.60 596.40 377 78.57 0.767
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 259.3 3
0237-0603
 585.14 603.71 378 75.26 0.839
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3153.4 3
0238-0600
 373.07 600.30 254 59.36 0.906
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 415.5 3
0239-0603
 923.84 602.96 310 66.43 0.883
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 771.5 3
0240-0604
 114.56 604.19 360 79.15 0.722
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1698.7 3
0241-0621
 767.20 622.36 953 139.78 0.613
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 251.9 3
0242-0608
 873.87 607.69 343 75.15 0.763
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 270.2 3
0243-0619
 887.26 619.02 411 81.15 0.784
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 233.9 3
0244-0617
 824.32 617.02 347 70.67 0.873
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 343.2 3
0245-0621
 358.11 621.50 605 95.40 0.835
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 252.5 3
0246-0620
 736.77 620.01 369 81.98 0.690
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2959.7 3
0247-0619
 389.04 619.47 348 72.08 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1206.2 3
0248-0626
 210.28 626.97 308 71.36 0.760
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 400.6 3
0249-0631
 692.35 631.02 357 79.98 0.701
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 691.6 3
0250-0632
 846.96 632.32 249 57.36 0.951
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 457.0 3
0251-0636
 620.94 636.44 368 73.01 0.867
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 703.7 3
0252-0638
 328.05 638.02 417 85.15 0.723
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 329.4 3
0253-0642
 35.07 642.13 314 71.01 0.782
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 237.3 3
0254-0643
 168.48 643.62 337 71.50 0.828
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 668.1 3
0255-0645
 212.27 643.20 363 77.60 0.758
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 235.8 3
0256-0650
 129.25 650.17 437 81.74 0.822
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3213.8 3
0257-0649
 798.87 649.68 206 73.50 0.479
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 381.4 3
0258-0654
 92.12 653.99 352 70.67 0.886
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 935.1 3
0259-0653
 558.56 653.05 375 73.84 0.864
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1368.9 3
0260-0653
 675.92 653.29 342 73.84 0.788