Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 335.6 4
0043-0390
 53.66 390.17 240 60.18 0.833
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 432.3 4
0044-0393
 267.09 393.23 290 67.26 0.806
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 454.3 4
0045-0401
 830.60 401.78 210 53.94 0.907
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3132.5 4
0046-0420
 198.06 419.82 219 57.46 0.834
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 687.7 4
0047-0424
 944.84 423.39 270 62.77 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 379.4 4
0048-0440
 392.04 441.07 265 65.84 0.768
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 569.2 4
0049-0447
 554.05 446.96 215 56.18 0.856
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2646.2 4
0050-0446
 249.81 446.92 191 53.11 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 381.4 4
0051-0477
 904.87 477.53 234 57.70 0.883
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 381.9 4
0052-0487
 80.59 487.68 262 62.77 0.836
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2920.6 4
0053-0502
 230.11 501.89 197 53.46 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4371.7 4
0054-0517
 391.63 517.07 253 75.60 0.556
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3159.8 4
0055-0521
 120.17 521.56 214 55.36 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 396.6 4
0056-0523
 287.43 523.01 270 63.60 0.839
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3098.6 4
0057-0530
 144.08 530.84 219 55.46 0.895
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 750.0 4
0058-0560
 596.96 558.50 392 91.74 0.585
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 690.1 4
0059-0564
 877.39 564.54 192 51.11 0.924
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2212.9 4
0060-0577
 810.69 577.11 389 74.43 0.882
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 509.0 4
0061-0591
 113.98 591.55 247 59.94 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 441.5 4
0062-0606
 659.17 606.68 297 78.57 0.605
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 464.7 4
0063-0617
 452.28 617.65 211 56.77 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 698.1 4
0064-0621
 193.27 621.27 221 60.43 0.761
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 684.2 4
0065-0637
 190.91 637.47 228 60.18 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 806.6 4
0066-0644
 397.00 643.71 282 63.36 0.883
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 703.2 4
0067-0653
 92.97 652.55 189 56.53 0.743