Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 368.2 7
0035-0134
 17.37 135.18 431 82.91 0.788
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 415.8 7
0036-0135
 649.93 135.03 457 80.33 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 394.3 7
0037-0137
 769.78 137.10 439 82.91 0.802
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 330.0 7
0038-0140
 523.99 140.31 478 84.33 0.845
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 413.6 7
0039-0142
 721.43 141.48 426 78.67 0.865
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2312.1 7
0040-0146
 451.98 146.47 778 109.74 0.812
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 387.9 7
0041-0146
 107.97 146.29 445 79.26 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 381.6 7
0042-0148
 135.96 147.29 444 84.33 0.785
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 343.5 7
0043-0149
 316.88 149.62 395 74.43 0.896
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 328.1 7
0044-0154
 781.79 154.36 461 87.15 0.763
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 261.0 7
0045-0156
 845.85 156.05 488 85.74 0.834
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 349.0 7
0046-0162
 363.17 161.47 444 84.67 0.778
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 399.7 7
0047-0166
 385.36 167.21 434 82.77 0.796
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1586.8 7
0048-0172
 184.53 172.77 562 92.81 0.820
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 440.5 7
0049-0173
 454.80 171.96 385 78.18 0.791
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 369.9 7
0050-0177
 288.22 177.19 491 92.81 0.716
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 328.5 7
0051-0178
 528.37 178.22 462 85.15 0.801
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 735.0 7
0052-0182
 51.09 181.36 409 81.05 0.782
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 494.9 7
0053-0194
 217.97 194.61 776 108.47 0.829
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 640.9 7
0054-0197
 36.31 198.74 416 81.15 0.794
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 416.5 7
0055-0194
 138.10 194.57 402 78.08 0.829
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 294.7 7
0056-0201
 825.33 201.22 458 81.50 0.867
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 271.4 7
0057-0208
 730.55 207.85 446 88.57 0.714
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 412.6 7
0058-0208
 651.04 208.27 465 82.33 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 491.1 7
0059-0209
 931.10 209.15 402 78.18 0.826