Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 517.1 1
0206-0632
 54.26 631.53 380 72.67 0.904
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 368.0 1
0207-0634
 156.18 634.61 438 77.84 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 405.8 1
0208-0632
 314.53 632.31 371 75.60 0.816
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 388.4 1
0209-0635
 337.73 634.71 468 90.18 0.723
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 469.1 1
0210-0639
 845.81 638.80 759 104.33 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 374.2 1
0211-0637
 182.83 636.98 452 85.40 0.779
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 337.0 1
0212-0638
 479.84 638.61 466 84.91 0.812
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 309.4 1
0213-0643
 360.80 643.00 528 91.84 0.787
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.8 1
0214-0643
 403.72 643.53 391 72.43 0.937
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 323.7 1
0215-0644
 797.68 644.62 380 74.43 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1547.1 1
0216-0648
 23.17 647.57 369 76.18 0.799
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 395.5 1
0217-0653
 447.13 653.51 845 118.02 0.762
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 358.1 1
0218-0650
 266.50 650.23 286 67.60 0.787
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1031.5 1
0219-0650
 606.93 650.53 444 79.26 0.888
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 678.2 1
0220-0654
 76.29 654.09 364 70.43 0.922
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 729.4 1
0221-0657
 213.13 657.27 388 76.08 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 279.0 1
0222-0660
 914.73 660.25 368 72.43 0.882
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 405.5 1
0223-0665
 714.94 664.98 405 77.01 0.858
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 386.6 1
0224-0666
 743.38 666.07 459 82.91 0.839
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3049.8 1
0225-0666
 648.30 666.58 374 70.43 0.948
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3108.7 1
0226-0675
 326.89 674.93 673 97.84 0.883
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 435.8 1
0227-0680
 772.33 679.99 356 70.43 0.902
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1376.2 1
0228-0688
 812.57 689.08 726 108.43 0.776
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 270.2 1
0229-0686
 489.12 685.84 367 75.74 0.804
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 705.2 1
0230-0687
 143.02 687.83 440 78.43 0.899