Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 350.8 5
0315-0546
321.13 546.71 242 57.94 0.906
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 348.9 5
0316-0548
678.45 548.70 313 65.70 0.911
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 271.4 5
0317-0546
741.53 546.09 268 60.18 0.930
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 275.2 5
0318-0551
553.24 551.57 308 68.43 0.827
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 230.4 5
0319-0551
99.70 551.78 270 60.77 0.919
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 235.2 5
0320-0553
231.82 553.69 305 70.08 0.780
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 206.0 5
0321-0553
22.92 553.49 287 63.01 0.908
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 216.9 5
0322-0559
495.91 559.76 300 70.28 0.763
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.1 5
0323-0559
389.91 558.86 287 63.11 0.905
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 227.9 5
0324-0559
821.93 559.31 314 67.36 0.870
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.6 5
0325-0559
248.37 560.21 257 63.36 0.805
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 299.6 5
0326-0562
168.02 561.67 248 62.77 0.791
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 271.3 5
0327-0564
702.70 564.62 264 60.53 0.906
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.2 5
0328-0567
510.91 566.78 301 69.11 0.792
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 332.4 5
0329-0564
315.06 563.80 239 59.36 0.852
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 269.4 5
0330-0566
736.12 565.92 242 57.36 0.924
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.6 5
0331-0569
150.59 569.48 302 65.01 0.898
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 442.7 5
0332-0567
677.72 567.46 254 60.53 0.871
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 211.3 5
0333-0571
274.35 571.63 273 66.67 0.772
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.4 5
0334-0576
918.32 576.50 354 70.18 0.903
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 283.6 5
0335-0575
342.32 575.76 263 64.28 0.800
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 359.4 5
0336-0577
429.97 576.35 383 73.26 0.897
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 241.3 5
0337-0578
632.34 578.02 292 65.94 0.844
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 193.6 5
0338-0575
859.79 575.79 290 71.36 0.716
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 273.5 5
0339-0580
67.90 580.53 477 87.74 0.779