Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2633.3 5
0155-0305
480.20 305.32 257 61.94 0.842
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2945.4 5
0156-0306
344.77 306.66 267 60.77 0.909
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 561.7 5
0157-0310
929.56 310.24 264 59.36 0.942
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3226.6 5
0158-0311
743.22 311.03 377 73.01 0.889
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3918.9 5
0159-0312
518.81 312.77 358 71.01 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1141.0 5
0160-0319
431.69 319.49 278 62.77 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2606.0 5
0161-0321
867.44 320.62 300 65.01 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5142.3 5
0162-0322
603.54 321.53 290 64.77 0.869
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3403.2 5
0163-0324
762.76 324.64 284 64.77 0.851
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1285.9 5
0164-0324
697.15 324.25 312 65.94 0.902
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4234.4 5
0165-0328
256.84 329.58 236 62.77 0.753
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1826.7 5
0166-0330
903.78 330.83 318 65.94 0.919
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2138.5 5
0167-0332
640.93 332.77 255 59.11 0.917
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4579.6 5
0168-0334
385.47 335.14 313 70.43 0.793
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3708.3 5
0169-0339
199.53 339.86 402 79.26 0.804
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3862.3 5
0170-0337
336.46 336.80 347 68.77 0.922
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 930.1 5
0171-0337
109.22 337.39 234 55.70 0.948
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3719.2 5
0172-0341
728.54 341.60 301 64.77 0.902
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4496.3 5
0173-0348
247.30 348.39 312 73.01 0.735
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3107.0 5
0174-0347
518.90 347.95 266 61.36 0.888
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3235.2 5
0175-0350
550.53 349.87 304 66.18 0.872
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1655.9 5
0176-0351
28.14 351.54 308 65.01 0.916
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 950.6 5
0177-0351
671.10 352.07 300 66.43 0.854
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2660.6 5
0178-0351
288.63 351.57 242 57.36 0.924
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2836.5 5
0179-0353
605.08 353.64 328 67.84 0.896