Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 954.4 4
0033-0067
812.44 67.35 244 59.01 0.880
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1352.7 4
0034-0078
318.23 77.48 282 65.01 0.838
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1419.7 4
0035-0080
447.27 80.88 269 61.01 0.908
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2752.0 4
0036-0083
227.30 83.62 238 57.70 0.898
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1057.2 4
0037-0088
414.50 87.90 319 68.43 0.856
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 339.5 4
0038-0090
269.16 90.06 247 59.70 0.871
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1321.8 4
0039-0091
381.61 91.52 302 64.77 0.905
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2075.3 4
0040-0094
159.26 95.10 397 74.08 0.909
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1324.2 4
0041-0092
54.99 92.34 244 59.11 0.877
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 12970.8 4
0042-0093
914.93 93.62 295 64.53 0.890
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1419.3 4
0043-0097
678.35 97.15 251 59.94 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2846.0 4
0044-0100
209.86 100.34 241 57.11 0.928
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2260.7 4
0045-0106
848.60 106.41 340 68.77 0.903
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 485.9 4
0046-0104
798.65 103.91 138 47.21 0.778
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 510.5 4
0047-0104
810.06 105.10 155 47.21 0.874
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2044.9 4
0048-0109
245.63 109.06 236 56.53 0.928
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2521.9 4
0049-0113
274.94 112.69 293 69.26 0.768
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1545.6 4
0050-0111
326.72 110.87 297 65.36 0.874
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 774.9 4
0051-0119
62.82 119.71 273 61.60 0.904
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1918.2 4
0052-0123
742.79 123.25 327 67.94 0.890
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1012.4 4
0053-0130
181.11 130.31 271 64.77 0.812
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2766.8 4
0054-0129
601.27 129.58 255 63.60 0.792
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 7277.2 4
0055-0131
483.49 131.95 328 116.47 0.304
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1040.0 4
0056-0131
714.45 131.62 301 67.01 0.842
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2304.1 4
0057-0133
237.78 133.58 318 66.18 0.912