Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.7 6
0017-0183
 491.89 183.08 192 56.43 0.758
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 303.5 6
0018-0187
 116.46 188.41 137 46.63 0.792
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.6 6
0019-0200
 117.38 199.99 117 41.80 0.842
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.1 6
0020-0220
 67.60 220.10 243 57.70 0.917
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 274.7 6
0021-0225
 588.78 225.28 263 64.43 0.796
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 269.8 6
0022-0261
 649.15 261.19 225 59.70 0.793
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 298.3 6
0023-0323
 648.24 322.86 229 59.94 0.801
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 304.3 6
0024-0328
 763.41 329.02 193 57.11 0.744
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 214.3 6
0025-0339
 100.89 339.06 230 57.94 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.3 6
0026-0347
 418.78 347.62 272 65.60 0.794
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.6 6
0027-0369
 308.09 369.43 309 66.43 0.880
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.0 6
0028-0373
 11.55 373.61 238 63.11 0.751
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 233.4 6
0029-0373
 577.93 372.96 231 62.63 0.740
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 238.9 6
0030-0403
 351.02 403.11 335 70.18 0.855
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 231.6 6
0031-0413
 837.42 412.60 249 59.36 0.888
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 258.1 6
0032-0418
 255.47 418.73 301 69.01 0.794
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 270.7 6
0033-0426
 394.41 426.17 186 53.46 0.818
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 232.5 6
0034-0432
 873.70 432.07 251 60.53 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 334.2 6
0035-0454
 596.99 454.12 299 82.91 0.547
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 293.9 6
0036-0486
 26.23 486.05 202 55.11 0.836
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 258.1 6
0037-0513
 579.88 512.79 289 64.53 0.872
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 243.1 6
0038-0542
 313.65 541.48 130 43.94 0.846
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.9 6
0039-0546
 303.22 547.24 144 45.11 0.889
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 325.8 6
0040-0554
 190.36 554.58 209 53.36 0.923
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 272.8 6
0041-0567
 183.14 566.73 172 51.11 0.827