Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 243.4 2
0126-0684
 931.88 683.94 264 62.53 0.849
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 252.3 2
0127-0694
 19.90 693.98 297 67.60 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 214.6 2
0128-0705
 744.81 704.87 312 65.84 0.904
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 254.0 2
0129-0706
 272.33 705.97 274 61.70 0.905
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 302.7 2
0130-0704
 664.77 704.13 313 65.36 0.921
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 260.4 3
0001-0050
 476.08 49.13 337 67.70 0.924
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 283.5 3
0002-0055
 782.98 55.56 261 61.11 0.878
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 336.4 3
0003-0061
 427.72 60.68 259 63.26 0.813
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 268.1 3
0004-0062
 393.22 62.02 308 63.36 0.964
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 267.0 3
0005-0065
 594.31 65.58 268 65.11 0.794
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.0 3
0006-0068
 325.54 68.02 284 62.77 0.906
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 278.1 3
0007-0074
 358.23 74.13 275 61.94 0.901
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 322.4 3
0008-0089
 435.52 89.44 268 60.77 0.912
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 202.2 3
0009-0095
 26.42 95.35 307 67.84 0.838
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 785.1 3
0010-0109
 138.49 108.04 205 67.26 0.570
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 364.5 3
0011-0121
 338.26 120.75 128 83.98 0.228
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 326.3 3
0012-0130
 591.11 130.17 181 52.87 0.814
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 232.9 3
0013-0135
 62.32 134.57 325 67.60 0.894
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 299.9 3
0014-0142
 436.10 142.21 309 66.53 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 263.5 3
0015-0139
 714.84 139.16 329 69.84 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 215.4 3
0016-0141
 34.55 140.24 322 71.01 0.802
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 398.4 3
0017-0151
 362.17 151.19 344 69.01 0.908
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 408.6 3
0018-0146
 686.46 146.15 203 58.28 0.751
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 268.1 3
0019-0162
 798.40 161.54 373 72.43 0.894
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 257.2 3
0020-0164
 305.82 164.51 279 61.94 0.914