Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 203.2 7
0005-0043
 46.37 43.18 436 80.08 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3173.5 7
0006-0046
 171.75 45.61 395 76.18 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 174.0 7
0007-0056
 779.49 56.58 762 105.40 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 209.3 7
0008-0053
 366.46 52.98 413 77.84 0.857
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 178.7 7
0009-0061
 736.50 60.83 821 110.33 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 181.6 7
0010-0059
 514.63 59.64 469 80.67 0.906
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 202.6 7
0011-0060
 622.88 60.55 447 81.01 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 212.5 7
0012-0062
 112.13 62.35 400 73.84 0.922
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 209.4 7
0013-0064
 827.38 64.14 397 73.01 0.936
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 214.6 7
0014-0065
 209.40 65.33 397 75.26 0.881
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 192.6 7
0015-0068
 474.31 68.46 445 77.84 0.923
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 193.5 7
0016-0073
 759.50 73.09 441 82.33 0.818
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 194.6 7
0017-0071
 25.96 71.16 395 74.43 0.896
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 189.3 7
0018-0075
 168.45 75.59 400 75.26 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.6 7
0019-0076
 649.96 75.19 460 84.91 0.802
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 200.2 7
0020-0076
 914.47 76.08 389 74.67 0.877
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 214.4 7
0021-0081
 412.73 81.20 410 77.01 0.869
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.9 7
0022-0081
 855.73 80.86 331 68.67 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 218.7 7
0023-0090
 806.94 90.08 426 77.50 0.891
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.6 7
0024-0097
 123.66 96.62 861 120.47 0.746
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 202.2 7
0025-0100
 724.72 101.48 808 111.30 0.820
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 195.6 7
0026-0096
 869.09 97.42 428 79.74 0.846
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 190.2 7
0027-0096
 384.45 96.22 436 77.26 0.918
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 189.1 7
0028-0096
 561.68 96.12 419 76.08 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 192.6 7
0029-0103
 764.31 103.27 401 74.08 0.918