Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.1 5
0102-0377
 235.32 377.50 785 116.95 0.721
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 237.8 5
0103-0385
 597.11 385.01 934 125.68 0.743
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 255.0 5
0104-0389
 304.30 388.70 858 119.68 0.753
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 199.1 5
0105-0383
 122.06 383.52 392 76.57 0.840
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 217.8 5
0106-0388
 549.09 386.78 494 88.57 0.791
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.5 5
0107-0388
 493.48 388.54 448 86.57 0.751
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 214.3 5
0108-0392
 464.63 392.49 458 82.91 0.837
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 207.9 5
0109-0398
 361.87 397.81 415 76.43 0.893
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 204.3 5
0110-0397
 729.47 397.47 422 77.50 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.6 5
0111-0402
 644.21 402.75 401 75.60 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 358.3 5
0112-0408
 25.84 408.78 427 77.50 0.893
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 191.3 5
0113-0412
 687.08 412.42 423 78.67 0.859
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 206.0 5
0114-0420
 131.98 419.92 301 71.11 0.748
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.0 5
0115-0420
 149.08 419.68 190 53.21 0.843
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 249.4 5
0116-0426
 598.50 426.01 414 77.50 0.866
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.0 5
0117-0428
 881.31 428.51 431 80.33 0.839
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 180.8 5
0118-0433
 743.77 432.91 402 76.67 0.859
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 200.0 5
0119-0436
 519.01 436.38 420 78.08 0.866
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 192.1 5
0120-0440
 203.93 440.29 462 83.84 0.826
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 198.2 5
0121-0442
 226.39 442.23 462 81.60 0.872
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.6 5
0122-0442
 259.99 441.96 399 75.50 0.880
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 195.4 5
0123-0442
 396.28 441.63 404 77.26 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 216.4 5
0124-0452
 828.09 451.93 481 81.01 0.921
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 199.1 5
0125-0454
 567.20 454.24 407 76.08 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.0 5
0126-0458
 60.38 458.16 396 77.26 0.834