Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 241.5 7
0169-0585
 567.84 585.96 426 78.67 0.865
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.3 7
0170-0587
 200.56 587.01 459 81.50 0.868
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 231.8 7
0171-0586
 381.16 586.27 433 80.91 0.831
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 300.6 7
0172-0602
 84.26 601.97 412 79.98 0.809
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 228.5 7
0173-0608
 779.71 607.96 426 77.26 0.897
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 325.8 7
0174-0610
 934.27 610.47 448 84.91 0.781
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 276.2 7
0175-0612
 143.13 612.04 417 84.57 0.733
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 269.8 7
0176-0613
 692.71 612.59 428 79.26 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 249.0 7
0177-0615
 439.99 614.55 437 80.08 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 240.4 7
0178-0622
 309.28 622.42 452 80.91 0.868
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 482.8 7
0179-0623
 216.34 623.50 469 83.26 0.850
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 238.0 7
0180-0634
 512.13 633.38 446 82.33 0.827
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 228.9 7
0181-0636
 62.85 636.45 401 76.67 0.857
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 524.8 7
0182-0643
 332.89 641.98 838 120.13 0.730
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.2 7
0183-0645
 399.66 644.48 449 81.84 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 256.0 7
0184-0647
 279.46 648.02 378 80.33 0.736
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 248.7 7
0185-0651
 639.67 650.98 435 79.01 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 338.9 7
0186-0651
 360.99 651.61 463 84.08 0.823
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 252.0 7
0187-0655
 250.20 654.26 469 86.57 0.786
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 206.8 7
0188-0659
 500.84 660.70 463 85.74 0.791
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 546.1 7
0189-0655
 433.42 656.14 379 75.70 0.831
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 266.8 7
0190-0660
 835.93 660.18 449 80.91 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 245.8 7
0191-0669
 391.30 670.27 462 88.81 0.736
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 220.5 7
0192-0667
 17.13 667.33 458 80.91 0.879
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 282.9 7
0193-0667
 193.70 667.19 437 79.50 0.869