Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 343.8 6
0143-0400
 846.75 399.69 373 72.43 0.894
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 194.3 6
0144-0404
 713.06 404.11 418 78.91 0.844
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 228.6 6
0145-0406
 671.17 405.85 361 71.26 0.893
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 232.6 6
0146-0413
 138.98 413.47 371 79.74 0.733
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 487.0 6
0147-0418
 488.43 418.69 409 76.08 0.888
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 250.0 6
0148-0420
 205.89 419.93 366 72.67 0.871
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 245.0 6
0149-0430
 813.04 431.09 381 73.26 0.892
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 291.7 6
0150-0431
 347.02 431.33 380 73.01 0.896
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.4 6
0151-0432
 434.56 432.26 372 75.26 0.825
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 289.0 6
0152-0433
 386.89 433.27 332 67.01 0.929
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 229.8 6
0153-0436
 534.23 436.43 387 74.43 0.878
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 216.6 6
0154-0436
 566.83 435.94 392 77.50 0.820
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 368.8 6
0155-0448
 223.60 447.98 776 111.20 0.789
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.0 6
0156-0444
 25.13 445.46 410 79.64 0.812
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 236.3 6
0157-0442
 879.80 442.45 368 73.01 0.867
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 592.7 6
0158-0453
 629.25 451.93 805 122.61 0.673
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 354.7 6
0159-0450
 286.83 449.76 498 109.40 0.523
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 281.7 6
0160-0455
 546.95 455.76 440 81.26 0.837
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 191.9 6
0161-0473
 695.65 471.41 849 123.44 0.700
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 225.2 6
0162-0463
 767.08 463.73 453 79.94 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 215.7 6
0163-0470
 934.85 469.19 674 102.33 0.809
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.3 6
0164-0464
 910.91 463.51 355 70.77 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 245.9 6
0165-0469
 376.31 469.53 372 70.18 0.949
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 299.8 6
0166-0471
 177.58 471.75 413 78.67 0.839
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 316.3 6
0167-0472
 54.05 472.63 343 69.01 0.905