Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 238.6 9
0160-0335
827.11 334.40 269 65.01 0.800
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 361.7 9
0161-0334
653.21 334.08 294 63.94 0.904
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 970.5 9
0162-0337
158.65 337.24 315 66.77 0.888
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 231.5 9
0163-0339
906.65 338.05 301 66.18 0.864
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 464.3 9
0164-0341
280.12 341.05 159 46.87 0.910
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 306.5 9
0165-0346
405.37 345.82 260 64.53 0.785
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 224.0 9
0166-0346
501.49 346.23 253 57.94 0.947
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 524.5 9
0167-0349
610.54 349.77 277 61.94 0.907
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 202.0 9
0168-0351
141.79 350.62 239 59.01 0.862
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 505.3 9
0169-0351
658.83 350.93 212 54.77 0.888
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 399.8 9
0170-0350
285.21 350.41 164 53.70 0.715
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 265.8 9
0171-0356
820.54 356.82 457 79.50 0.909
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 228.2 9
0172-0356
448.40 356.50 293 63.26 0.920
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 206.4 9
0173-0355
476.70 354.62 261 60.53 0.895
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 258.4 9
0174-0357
577.40 356.95 299 68.77 0.794
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 727.4 9
0175-0358
238.80 359.38 238 58.18 0.883
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 210.5 9
0176-0357
419.39 357.22 250 59.70 0.882
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 486.1 9
0177-0361
555.88 361.12 311 71.01 0.775
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 267.7 9
0178-0361
65.88 360.72 247 58.18 0.917
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 206.6 9
0179-0361
19.97 360.70 264 61.11 0.888
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 204.5 9
0180-0361
132.83 362.58 260 61.84 0.854
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 176.4 9
0181-0364
345.75 364.55 320 67.84 0.874
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 5103.6 9
0182-0362
689.31 362.03 220 55.11 0.910
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 631.6 9
0183-0365
192.24 365.31 356 69.60 0.924
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 838.9 9
0184-0369
298.99 368.75 288 61.36 0.961