Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 195.8 5
0188-0349
 287.08 348.91 530 90.08 0.821
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 241.0 5
0189-0348
 528.05 348.36 309 64.77 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1323.5 5
0190-0349
 862.51 349.51 310 65.70 0.903
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 567.1 5
0191-0350
 111.55 349.91 286 63.94 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 187.5 5
0192-0351
 185.97 351.54 249 59.11 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 391.2 5
0193-0351
 484.60 351.12 260 59.46 0.924
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 236.9 5
0194-0351
 626.22 351.50 270 60.77 0.919
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 212.1 5
0195-0355
 259.67 355.61 272 62.43 0.877
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 475.0 5
0196-0355
 379.09 355.60 217 55.94 0.871
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 203.1 5
0197-0357
 925.07 357.27 292 67.01 0.817
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 302.3 5
0198-0359
 132.19 358.49 242 57.36 0.924
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 780.4 5
0199-0358
 402.98 357.83 219 56.53 0.861
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 217.0 5
0200-0360
 231.80 360.52 300 66.53 0.852
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 193.0 5
0201-0363
 330.82 363.80 293 67.84 0.800
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3491.2 5
0202-0362
 570.58 361.86 347 68.43 0.931
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 576.3 5
0203-0363
 801.77 362.94 302 65.11 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 453.5 5
0204-0362
 692.19 362.38 273 62.77 0.871
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 497.6 5
0205-0362
 455.04 362.52 265 59.94 0.927
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 321.9 5
0206-0367
 753.07 367.37 195 53.70 0.850
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 201.6 5
0207-0370
 250.40 370.10 267 60.77 0.909
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 418.4 5
0208-0367
 489.65 367.26 252 61.70 0.832
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.4 5
0209-0370
 38.62 370.24 269 61.60 0.891
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 203.7 5
0210-0371
 65.11 370.95 267 63.60 0.830
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 446.3 5
0211-0379
 16.47 379.21 528 92.33 0.778
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1200.8 5
0212-0374
 897.56 373.81 358 70.43 0.907