Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2141.9 2
0046-0122
 931.96 121.35 274 80.57 0.530
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2521.7 2
0047-0123
 516.32 123.01 286 63.36 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2993.6 2
0048-0122
 563.25 122.04 263 59.11 0.946
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 742.6 2
0049-0128
 97.77 128.00 262 60.53 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3549.9 2
0050-0129
 42.10 128.79 231 55.36 0.947
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1707.5 2
0051-0128
 207.62 128.42 259 59.60 0.916
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1693.0 2
0052-0133
 604.70 133.19 360 70.43 0.912
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1351.7 2
0053-0130
 759.25 130.71 251 58.18 0.932
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1952.3 2
0054-0136
 72.94 136.44 208 54.28 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3850.8 2
0055-0137
 550.32 136.78 274 61.36 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 7825.2 2
0056-0137
 777.44 137.14 222 55.11 0.918
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 8595.0 2
0057-0137
 794.18 136.40 218 53.70 0.950
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1589.3 2
0058-0145
 727.37 145.32 444 78.18 0.913
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 4541.8 2
0059-0142
 874.27 142.34 224 55.36 0.919
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2891.9 2
0060-0144
 934.42 143.85 249 58.77 0.906
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 802.5 2
0061-0148
 155.76 148.52 292 65.84 0.846
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2825.7 2
0062-0149
 33.25 149.15 222 54.77 0.930
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1321.6 2
0063-0150
 106.39 149.81 237 57.36 0.905
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1638.6 2
0064-0155
 200.80 155.72 393 73.60 0.912
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2878.9 2
0065-0155
 471.21 154.30 254 59.01 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3852.0 2
0066-0154
 558.32 154.44 292 63.01 0.924
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 5371.5 2
0067-0154
 254.47 153.92 308 67.36 0.853
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1253.7 2
0068-0160
 620.04 160.01 404 74.43 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3113.2 2
0069-0164
 917.28 164.36 356 70.43 0.902
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2703.4 2
0070-0164
 175.67 164.23 215 55.36 0.882