Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 335.3 1
0074-0152
624.74 152.68 246 59.11 0.885
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 278.3 1
0075-0156
784.87 156.36 297 65.60 0.867
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 272.0 1
0076-0159
884.42 159.25 315 70.43 0.798
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 248.8 1
0077-0160
127.31 160.31 303 65.36 0.891
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 381.8 1
0078-0162
305.18 162.32 272 63.60 0.845
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 344.4 1
0079-0168
288.46 168.54 311 67.36 0.861
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 190.5 1
0080-0171
832.81 171.77 291 68.18 0.787
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 375.5 1
0081-0174
42.34 174.21 262 63.36 0.820
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 368.9 1
0082-0170
729.21 170.50 271 61.94 0.888
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 249.5 1
0083-0173
590.40 173.08 296 63.36 0.927
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 424.1 1
0084-0178
83.14 178.73 315 65.01 0.937
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 436.1 1
0085-0177
171.65 177.29 246 57.36 0.940
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 339.0 1
0086-0182
691.21 182.38 284 63.36 0.889
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 289.8 1
0087-0186
447.87 186.68 253 59.36 0.902
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 477.7 1
0088-0188
242.39 187.62 194 51.94 0.904
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 577.9 1
0089-0193
516.56 193.62 611 101.98 0.738
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 299.6 1
0090-0190
635.08 190.19 316 70.43 0.801
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.0 1
0091-0192
872.76 192.02 284 61.36 0.948
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 303.7 1
0092-0193
736.41 192.47 313 65.60 0.914
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 362.2 1
0093-0194
386.17 193.83 254 58.53 0.932
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 262.9 1
0094-0194
823.62 193.96 316 67.84 0.863
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.7 1
0095-0196
920.81 196.20 290 64.43 0.878
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 285.6 1
0096-0196
427.88 196.19 297 65.01 0.883
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 225.7 1
0097-0201
117.09 201.51 288 64.28 0.876
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 303.4 1
0098-0201
53.21 201.78 281 60.77 0.956