Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5847.6 8
0062-0701
 133.33 701.42 195 51.70 0.917
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 153.5 8
0063-0709
 842.07 709.27 198 51.70 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 278.9 9
0001-0034
 31.77 34.33 273 65.01 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 268.0 9
0002-0034
 411.40 35.09 286 64.77 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 623.2 9
0003-0040
 489.21 39.94 280 65.84 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 470.5 9
0004-0049
 727.83 49.69 258 61.11 0.868
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 246.5 9
0005-0071
 43.56 71.40 286 64.28 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1943.1 9
0006-0103
 476.99 103.18 279 61.36 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 268.7 9
0007-0106
 354.74 106.26 246 59.60 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 617.2 9
0008-0118
 570.68 118.15 240 60.53 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3387.0 9
0009-0145
 347.01 145.15 199 53.11 0.886
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1040.2 9
0010-0148
 694.70 149.05 194 59.36 0.692
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1278.2 9
0011-0150
 125.25 150.20 225 59.46 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3180.5 9
0012-0157
 97.26 157.47 266 59.11 0.957
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 243.7 9
0013-0183
 323.97 183.54 246 61.60 0.815
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1859.2 9
0014-0194
 251.35 194.46 224 57.36 0.856
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 301.9 9
0015-0206
 858.14 205.90 261 62.18 0.848
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 742.6 9
0016-0210
 14.17 209.59 216 55.60 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2732.8 9
0017-0211
 377.37 210.79 172 54.04 0.740
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5134.7 9
0018-0224
 388.18 223.96 199 56.53 0.783
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2499.7 9
0019-0235
 80.57 235.54 201 56.18 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 501.3 9
0020-0244
 687.08 243.72 274 63.36 0.858
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 274.0 9
0021-0246
 299.45 245.54 343 72.43 0.822
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 321.9 9
0022-0255
 445.90 255.26 242 57.94 0.906
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1972.4 9
0023-0268
 478.14 267.59 245 58.77 0.891