Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1027.5 4
0036-0350
 928.55 349.11 162 51.94 0.755
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 854.7 4
0037-0355
 938.44 356.34 139 44.28 0.891
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2122.9 4
0038-0361
 218.64 361.70 202 57.11 0.778
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 267.3 4
0039-0373
 278.48 373.34 189 51.46 0.897
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 375.3 4
0040-0382
 801.04 382.03 194 55.70 0.786
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 867.1 4
0041-0388
 284.57 388.10 256 62.53 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1643.6 4
0042-0391
 415.82 392.06 295 67.36 0.817
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 397.0 4
0043-0390
 53.66 390.17 240 60.18 0.833
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1155.6 4
0044-0393
 267.09 393.23 290 67.26 0.806
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 558.0 4
0045-0401
 830.60 401.78 210 53.94 0.907
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2170.3 4
0046-0420
 198.06 419.82 219 57.46 0.834
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 645.1 4
0047-0424
 944.84 423.39 270 62.77 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1787.9 4
0048-0440
 392.04 441.07 265 65.84 0.768
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1724.1 4
0049-0447
 554.05 446.96 215 56.18 0.856
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1890.1 4
0050-0446
 249.81 446.92 191 53.11 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 244.8 4
0051-0477
 904.87 477.53 234 57.70 0.883
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 261.7 4
0052-0487
 80.59 487.68 262 62.77 0.836
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1525.2 4
0053-0502
 230.11 501.89 197 53.46 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 362.3 4
0054-0517
 391.63 517.07 253 75.60 0.556
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 379.8 4
0055-0521
 120.17 521.56 214 55.36 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 6482.6 4
0056-0523
 287.43 523.01 270 63.60 0.839
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 389.4 4
0057-0530
 144.08 530.84 219 55.46 0.895
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2032.8 4
0058-0560
 596.96 558.50 392 91.74 0.585
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 301.8 4
0059-0564
 877.39 564.54 192 51.11 0.924
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 223.8 4
0060-0577
 810.69 577.11 389 74.43 0.882