Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2492.2 8
0081-0390
 343.64 390.06 371 73.84 0.855
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 515.7 8
0082-0386
 263.47 386.26 253 61.11 0.851
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 523.9 8
0083-0391
 908.93 390.40 404 73.60 0.937
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 642.4 8
0084-0391
 611.42 391.77 274 69.50 0.713
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 243.8 8
0085-0394
 117.33 393.86 319 67.60 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 906.0 8
0086-0399
 228.50 398.65 247 60.53 0.847
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 297.3 8
0087-0408
 842.54 408.45 219 54.63 0.922
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1099.2 8
0088-0416
 556.27 416.95 354 69.60 0.918
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4797.3 8
0089-0420
 159.54 420.41 357 71.50 0.878
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1396.7 8
0090-0423
 731.25 422.91 279 63.01 0.883
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1054.5 8
0091-0428
 804.60 428.93 253 61.36 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 940.1 8
0092-0436
 487.98 436.01 325 66.77 0.916
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 220.8 8
0093-0438
 450.52 438.13 320 69.84 0.824
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1425.3 8
0094-0441
 747.09 440.58 241 61.11 0.811
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 234.6 8
0095-0449
 897.52 449.38 338 69.84 0.871
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 275.2 8
0096-0449
 761.52 449.23 256 62.53 0.823
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5274.0 8
0097-0454
 127.49 454.78 354 70.18 0.903
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 260.5 8
0098-0459
 837.61 458.78 342 69.84 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 461.0 8
0099-0464
 52.51 464.29 258 63.01 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 350.8 8
0100-0475
 743.13 475.27 277 63.01 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 384.2 8
0101-0501
 414.64 501.00 335 69.60 0.869
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 725.0 8
0102-0507
 566.44 507.64 333 69.60 0.864
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 303.5 8
0103-0514
 374.99 514.14 306 65.94 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1342.2 8
0104-0513
 334.32 513.20 159 48.53 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1370.9 8
0105-0524
 327.91 524.49 200 53.94 0.864