Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 215.2 1
0065-0349
 610.56 349.57 361 71.60 0.885
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 325.0 1
0066-0349
 313.63 348.58 294 67.26 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 816.0 1
0067-0352
 260.23 352.27 281 67.11 0.784
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 309.0 1
0068-0354
 442.75 354.67 287 63.94 0.882
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 244.8 1
0069-0363
 480.92 363.36 340 69.60 0.882
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1254.9 1
0070-0367
 299.40 366.87 304 65.36 0.894
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 215.5 1
0071-0365
 834.10 365.25 322 69.01 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1892.1 1
0072-0368
 527.07 367.61 294 64.18 0.897
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1274.9 1
0073-0382
 713.27 382.72 345 71.50 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 213.1 1
0074-0384
 173.41 384.36 362 72.77 0.859
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1104.9 1
0075-0391
 638.78 392.22 333 68.18 0.900
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 228.0 1
0076-0405
 573.29 404.87 344 69.60 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 482.3 1
0077-0406
 83.10 406.45 337 72.08 0.815
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2967.6 1
0078-0409
 594.00 410.00 382 76.43 0.822
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1455.4 1
0079-0416
 504.68 415.87 285 67.01 0.798
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1743.4 1
0080-0418
 699.52 417.20 307 69.50 0.799
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 447.8 1
0081-0418
 203.10 417.77 265 61.36 0.885
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 209.9 1
0082-0428
 690.61 428.69 321 68.91 0.849
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 195.9 1
0083-0435
 651.67 435.56 372 72.67 0.885
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 233.9 1
0084-0437
 450.85 437.17 367 70.77 0.921
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 523.9 1
0085-0435
 195.15 435.23 264 61.36 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 672.0 1
0086-0441
 111.14 440.70 368 73.84 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 225.4 1
0087-0436
 937.32 436.53 267 61.70 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1158.3 1
0088-0444
 253.50 444.16 346 118.71 0.309
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 772.3 1
0089-0455
 397.40 455.25 368 71.01 0.917