Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 349.6 9
0042-0181
 472.02 180.70 441 80.08 0.864
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 261.4 9
0043-0183
 320.29 182.67 391 75.26 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1027.9 9
0044-0190
 33.68 190.27 339 69.01 0.894
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2681.0 9
0045-0190
 662.58 189.98 368 74.43 0.835
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 496.8 9
0046-0201
 870.57 199.09 990 127.54 0.765
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 436.3 9
0047-0198
 642.58 198.23 392 75.60 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 745.8 9
0048-0198
 62.31 198.18 337 69.26 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 381.8 9
0049-0204
 137.30 204.06 380 75.84 0.830
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1855.9 9
0050-0204
 931.35 203.84 337 68.18 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 214.4 9
0051-0207
 116.09 206.71 381 75.01 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1399.7 9
0052-0218
 365.71 218.11 398 77.50 0.833
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 235.0 9
0053-0223
 202.20 223.22 396 76.08 0.860
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1244.2 9
0054-0235
 446.43 235.83 404 77.74 0.840
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 748.7 9
0055-0234
 300.74 233.79 385 75.26 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1445.7 9
0056-0237
 852.00 237.08 388 73.01 0.915
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 496.9 9
0057-0246
 898.77 247.10 689 110.37 0.711
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1024.6 9
0058-0245
 510.67 246.13 412 75.26 0.914
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1264.5 9
0059-0250
 571.60 249.66 391 73.01 0.922
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2192.2 9
0060-0254
 95.42 254.05 353 68.18 0.954
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 488.5 9
0061-0261
 257.36 261.65 412 80.43 0.800
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1431.6 9
0062-0264
 925.97 264.27 369 71.01 0.920
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 275.0 9
0063-0267
 276.08 268.10 156 48.87 0.821
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5828.4 9
0064-0295
 116.10 293.14 552 94.23 0.781
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1180.5 9
0065-0296
 865.27 296.47 330 67.94 0.898
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 885.6 9
0066-0308
 601.08 308.01 785 119.10 0.695