Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1090.2 5
0136-0500
 732.05 500.30 421 78.91 0.850
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 299.6 5
0137-0510
 41.05 510.31 392 74.67 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 193.2 5
0138-0511
 901.81 511.56 447 79.50 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 321.3 5
0139-0514
 171.32 513.56 408 78.57 0.831
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 211.9 5
0140-0515
 814.29 515.03 404 77.26 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 255.0 5
0141-0517
 762.03 517.57 409 75.84 0.894
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 451.8 5
0142-0518
 524.67 518.08 411 77.26 0.865
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 710.0 5
0143-0530
 669.23 530.32 405 76.67 0.866
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 300.6 5
0144-0535
 381.83 535.94 398 75.01 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 516.0 5
0145-0537
 450.65 536.69 413 79.26 0.826
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 194.9 5
0146-0540
 496.57 540.79 433 77.01 0.917
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1063.4 5
0147-0543
 575.29 542.93 427 80.33 0.832
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 612.6 5
0148-0551
 803.26 551.15 813 113.05 0.799
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 255.5 5
0149-0551
 922.87 550.54 310 66.18 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 292.2 5
0150-0562
 119.28 562.25 412 78.33 0.844
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1300.3 5
0151-0565
 243.17 565.20 397 74.91 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 240.3 5
0152-0565
 856.86 565.48 401 73.84 0.924
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 638.6 5
0153-0572
 309.73 571.73 409 79.50 0.813
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 232.1 5
0154-0576
 918.68 577.10 561 89.15 0.887
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 253.1 5
0155-0579
 350.68 579.12 403 75.50 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 519.5 5
0156-0580
 58.30 579.86 385 74.67 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 694.3 5
0157-0584
 284.01 584.18 751 105.64 0.846
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1898.2 5
0158-0590
 578.60 590.09 424 82.08 0.791
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 245.0 5
0159-0593
 777.12 593.57 371 75.74 0.813
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 294.0 5
0160-0595
 468.12 594.79 435 77.84 0.902