Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 686.7 3
0048-0194
 31.71 193.70 709 107.74 0.768
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 252.8 3
0049-0193
 307.19 192.85 192 57.11 0.740
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 506.4 3
0050-0210
 253.54 209.53 363 71.26 0.898
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1664.2 3
0051-0214
 662.03 214.98 374 73.84 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 356.9 3
0052-0225
 202.17 225.33 359 70.67 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 502.8 3
0053-0233
 894.48 232.88 741 113.20 0.727
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 369.4 3
0054-0226
 681.37 224.84 416 77.74 0.865
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 700.2 3
0055-0226
 622.13 226.02 368 72.43 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 223.5 3
0056-0230
 231.70 229.44 406 77.50 0.849
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3552.5 3
0057-0229
 432.85 229.64 372 71.84 0.906
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3102.4 3
0058-0233
 377.23 232.95 380 72.77 0.902
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 890.5 3
0059-0238
 698.28 238.85 444 79.15 0.891
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1122.5 3
0060-0239
 113.51 238.68 366 72.67 0.871
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5722.8 3
0061-0248
 500.11 247.44 356 70.67 0.896
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 246.4 3
0062-0251
 752.47 251.41 385 75.26 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 388.3 3
0063-0256
 162.63 255.61 376 76.91 0.799
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3033.9 3
0064-0276
 640.98 276.20 848 126.02 0.671
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 653.2 3
0065-0267
 864.53 267.31 398 74.43 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 202.0 3
0066-0277
 682.08 277.03 520 85.50 0.894
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 898.2 3
0067-0280
 937.73 280.03 369 72.43 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3288.4 3
0068-0282
 619.21 283.03 418 80.67 0.807
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 343.3 3
0069-0283
 243.07 283.34 402 77.50 0.841
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.4 3
0070-0286
 502.83 286.56 398 75.01 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 203.1 3
0071-0297
 168.63 296.89 692 98.33 0.899
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1095.1 3
0072-0293
 818.20 293.63 379 73.01 0.893