Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 223.2 1
0182-0606
 347.15 606.58 459 81.26 0.874
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 306.3 1
0183-0618
 391.24 618.44 390 73.84 0.899
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2720.7 1
0184-0620
 797.93 620.38 546 91.60 0.818
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 477.8 1
0185-0637
 64.60 637.63 890 119.30 0.786
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 235.9 1
0186-0631
 719.70 631.93 467 81.60 0.881
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 490.7 1
0187-0631
 176.97 631.35 403 76.67 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 284.5 1
0188-0631
 697.91 631.62 408 74.53 0.923
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3790.8 1
0189-0632
 215.71 632.05 430 79.50 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 185.9 1
0190-0637
 18.73 637.73 398 78.91 0.803
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 230.0 1
0191-0635
 553.23 634.85 420 77.26 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 474.6 1
0192-0640
 93.70 640.64 451 79.74 0.891
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 324.0 1
0193-0639
 869.53 638.74 414 76.67 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 824.9 1
0194-0643
 330.43 643.90 423 76.43 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1753.9 1
0195-0655
 426.54 655.75 880 119.05 0.780
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 343.7 1
0196-0651
 773.20 651.09 440 77.84 0.913
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 290.8 1
0197-0655
 649.45 655.53 469 82.08 0.875
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 724.5 1
0198-0655
 388.48 654.75 422 78.67 0.857
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 294.4 1
0199-0654
 481.44 654.30 409 75.01 0.913
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 230.8 1
0200-0660
 517.44 660.08 452 80.91 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 557.4 1
0201-0661
 932.46 661.06 366 71.60 0.897
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 476.7 1
0202-0662
 256.71 662.73 437 83.60 0.786
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 250.4 1
0203-0666
 826.30 664.91 413 79.15 0.828
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 449.0 1
0204-0678
 175.43 678.36 407 77.11 0.860
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 281.3 1
0205-0678
 554.22 678.45 450 81.26 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 219.0 1
0206-0678
 633.42 677.98 463 82.08 0.864