Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 319.0 7
0103-0372
 28.17 372.53 757 109.84 0.788
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 818.1 7
0104-0379
 122.49 379.22 502 90.18 0.776
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4334.9 7
0105-0383
 142.26 381.40 342 74.43 0.776
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 203.4 7
0106-0384
 326.48 383.91 537 91.15 0.812
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 472.4 7
0107-0385
 592.79 385.21 478 82.91 0.874
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2131.5 7
0108-0390
 198.62 390.20 396 77.26 0.834
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1037.4 7
0109-0390
 648.07 390.05 430 79.26 0.860
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 212.1 7
0110-0392
 353.79 391.83 524 97.64 0.691
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 743.6 7
0111-0395
 155.03 396.14 484 86.81 0.807
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 221.3 7
0112-0393
 388.35 393.40 457 80.91 0.877
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 676.7 7
0113-0401
 715.27 400.99 439 83.74 0.787
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1654.7 7
0114-0401
 910.08 400.87 410 77.26 0.863
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 977.6 7
0115-0402
 80.39 401.50 410 78.08 0.845
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 202.4 7
0116-0408
 115.91 409.20 851 116.95 0.782
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1149.7 7
0117-0406
 47.71 407.15 428 79.60 0.849
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 199.9 7
0118-0417
 672.49 417.03 472 85.50 0.811
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2909.4 7
0119-0419
 258.24 419.29 418 75.26 0.928
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3029.0 7
0120-0419
 852.42 418.87 438 77.84 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1188.5 7
0121-0422
 462.95 422.00 463 84.57 0.814
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 245.7 7
0122-0428
 737.20 428.53 799 106.23 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1283.5 7
0123-0426
 357.00 426.46 420 78.91 0.848
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1403.0 7
0124-0433
 207.82 432.94 401 76.67 0.857
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1032.1 7
0125-0439
 548.38 439.23 853 114.71 0.815
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 478.8 7
0126-0445
 130.87 445.13 436 81.15 0.832
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 241.7 7
0127-0446
 872.69 446.15 435 79.50 0.865