Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 832.7 6
0127-0350
 932.91 350.68 699 110.81 0.715
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 277.1 6
0128-0353
 876.46 352.96 385 73.01 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 247.4 6
0129-0358
 727.31 358.52 378 72.43 0.906
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1222.0 6
0130-0371
 408.14 370.44 795 118.02 0.717
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 321.0 6
0131-0366
 226.35 365.77 908 116.33 0.843
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 394.7 6
0132-0372
 118.56 372.22 342 68.77 0.909
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1609.3 6
0133-0380
 171.75 380.43 359 71.01 0.895
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 260.2 6
0134-0381
 686.19 380.93 378 72.43 0.906
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 412.8 6
0135-0386
 356.86 385.91 362 71.84 0.881
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 491.1 6
0136-0386
 898.63 385.61 350 70.43 0.887
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4329.0 6
0137-0389
 582.02 388.69 403 77.26 0.849
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 963.4 6
0138-0393
 465.08 393.73 397 76.33 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 434.1 6
0139-0396
 531.89 396.65 383 73.60 0.889
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2269.0 6
0140-0398
 404.18 398.80 397 75.26 0.881
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 331.7 6
0141-0399
 263.14 398.89 376 72.43 0.901
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 268.1 6
0142-0406
 755.66 406.81 828 112.81 0.818
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 246.1 6
0143-0400
 846.75 399.69 373 72.43 0.894
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 240.2 6
0144-0404
 713.06 404.11 418 78.91 0.844
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1258.2 6
0145-0406
 671.17 405.85 361 71.26 0.893
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 608.7 6
0146-0413
 138.98 413.47 371 79.74 0.733
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1050.3 6
0147-0418
 488.43 418.69 409 76.08 0.888
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 587.2 6
0148-0420
 205.89 419.93 366 72.67 0.871
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 257.2 6
0149-0430
 813.04 431.09 381 73.26 0.892
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 289.8 6
0150-0431
 347.02 431.33 380 73.01 0.896
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 597.0 6
0151-0432
 434.56 432.26 372 75.26 0.825