Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 293.1 8
0308-0503
 162.81 503.82 231 57.94 0.865
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 484.4 8
0309-0502
 252.53 502.28 254 61.11 0.855
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 323.5 8
0310-0502
 720.89 503.30 199 55.11 0.823
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 334.6 8
0311-0506
 118.28 506.07 213 55.36 0.874
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 304.8 8
0312-0506
 213.48 506.27 246 58.53 0.902
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.5 8
0313-0505
 513.95 505.20 270 61.36 0.901
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 213.7 8
0314-0512
 391.39 512.53 273 65.26 0.806
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.5 8
0315-0508
 573.78 508.38 197 52.63 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 394.2 8
0316-0513
 459.25 513.30 269 62.77 0.858
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 461.2 8
0317-0516
 279.40 515.67 340 72.91 0.804
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 358.7 8
0318-0517
 34.47 517.38 330 67.01 0.923
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 330.0 8
0319-0519
 191.56 519.29 327 67.60 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 277.7 8
0320-0520
 598.95 520.02 222 58.43 0.817
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 212.2 8
0321-0521
 490.02 521.05 227 55.70 0.919
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.3 8
0322-0523
 544.13 524.15 269 79.01 0.541
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.1 8
0323-0524
 373.61 524.93 246 58.77 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 349.9 8
0324-0524
 249.55 524.74 219 54.87 0.914
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 329.1 8
0325-0528
 587.81 528.57 220 57.26 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 365.6 8
0326-0528
 661.83 528.57 227 57.11 0.875
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 484.9 8
0327-0529
 741.26 528.96 331 66.18 0.950
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 418.5 8
0328-0529
 265.76 529.33 168 49.11 0.875
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 394.5 8
0329-0530
 886.67 530.57 195 53.36 0.861
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 336.1 8
0330-0536
 475.34 535.73 334 73.36 0.780
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 323.4 8
0331-0532
 693.26 532.27 262 62.77 0.836
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 292.1 8
0332-0535
 904.34 535.08 217 57.11 0.836