Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 242.2 3
0128-0263
942.26 262.18 227 57.70 0.857
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 247.1 3
0129-0266
729.09 265.65 222 54.53 0.938
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 612.6 3
0130-0271
632.23 271.21 387 98.47 0.502
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 294.1 3
0131-0276
877.95 276.41 209 53.46 0.919
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 504.9 3
0132-0280
532.20 280.58 357 69.01 0.942
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 302.5 3
0133-0283
898.83 283.28 321 65.60 0.937
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 596.1 3
0134-0287
309.24 286.78 291 63.60 0.904
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 252.5 3
0135-0290
800.71 289.32 280 63.36 0.877
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 387.3 3
0136-0289
858.29 289.80 228 56.53 0.897
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 746.1 3
0137-0293
677.33 293.41 287 63.94 0.882
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1660.6 3
0138-0291
459.36 291.79 320 66.77 0.902
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 196.4 3
0139-0297
354.38 297.64 401 79.50 0.797
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 299.5 3
0140-0294
757.01 294.27 198 55.11 0.819
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 188.6 3
0141-0299
576.94 298.66 443 79.60 0.879
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1062.5 3
0142-0298
265.74 298.65 267 61.11 0.898
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 4680.5 3
0143-0299
72.18 299.74 253 58.77 0.921
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 234.9 3
0144-0304
788.08 303.48 234 59.01 0.844
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2434.5 3
0145-0311
518.79 311.58 293 63.84 0.903
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1548.6 3
0146-0311
866.10 311.35 321 66.18 0.921
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 236.5 3
0147-0313
114.14 313.03 312 67.84 0.852
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 197.6 3
0148-0316
281.76 314.32 322 72.77 0.764
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 429.6 3
0149-0314
216.74 314.24 240 57.36 0.917
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 294.9 3
0150-0316
462.68 315.95 374 71.84 0.911
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2063.5 3
0151-0312
671.78 312.33 293 66.77 0.826
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3258.0 3
0152-0319
404.30 319.33 365 90.33 0.562