Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 658.2 8
0265-0433
292.44 431.71 270 62.77 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 290.5 8
0266-0433
463.81 433.41 344 70.77 0.863
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 222.6 8
0267-0440
612.69 440.34 286 66.18 0.820
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 209.8 8
0268-0440
376.98 439.41 296 67.36 0.820
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 258.0 8
0269-0441
917.64 440.86 239 59.36 0.852
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1202.3 8
0270-0441
571.71 441.23 324 67.01 0.907
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 343.5 8
0271-0448
119.36 447.66 377 73.60 0.875
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1342.7 8
0272-0444
709.38 444.02 214 53.70 0.933
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 445.5 8
0273-0446
210.10 445.78 171 49.70 0.870
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 476.6 8
0274-0450
251.93 449.17 467 84.33 0.825
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 437.5 8
0275-0445
284.42 445.95 125 42.53 0.869
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 257.4 8
0276-0447
758.43 447.39 273 63.36 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3347.9 8
0277-0447
510.30 448.26 285 65.60 0.832
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 412.6 8
0278-0451
197.74 451.73 214 54.77 0.896
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1140.8 8
0279-0452
679.58 452.71 229 56.53 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 253.6 8
0280-0455
414.52 455.68 259 65.26 0.764
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1011.4 8
0281-0458
645.04 457.69 235 57.70 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 247.0 8
0282-0461
893.09 460.31 237 61.11 0.797
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 299.0 8
0283-0461
806.05 461.06 265 61.26 0.888
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 271.6 8
0284-0460
849.37 459.94 300 73.26 0.703
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 742.6 8
0285-0467
482.18 466.42 278 65.36 0.818
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 578.4 8
0286-0467
502.80 466.67 202 52.77 0.912
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 474.6 8
0287-0468
175.68 468.11 131 44.39 0.836
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1349.4 8
0288-0469
188.14 470.18 220 55.46 0.899
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 401.3 8
0289-0470
164.58 470.70 133 44.87 0.830