Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 380.4 6
0398-0700
708.19 700.42 393 75.01 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 208.5 6
0399-0699
725.25 699.07 256 59.70 0.903
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 8015.7 6
0400-0703
517.50 703.30 205 54.53 0.866
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1292.2 6
0401-0706
576.37 706.19 196 53.94 0.846
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 343.8 6
0402-0707
116.69 707.16 302 66.43 0.860
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 286.9 6
0403-0709
376.77 708.80 241 61.70 0.796
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 804.1 7
0001-0026
81.39 26.79 137 43.94 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3454.1 7
0002-0029
247.78 28.87 248 59.11 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1761.5 7
0003-0028
824.82 27.21 222 55.94 0.891
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 559.0 7
0004-0033
560.84 33.13 299 64.77 0.896
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1620.3 7
0005-0031
711.12 31.85 252 59.11 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2021.8 7
0006-0036
632.02 36.41 452 80.08 0.886
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 659.8 7
0007-0036
390.73 36.07 154 54.28 0.657
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 217.9 7
0008-0040
52.67 39.62 298 66.18 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 507.2 7
0009-0037
403.64 36.79 145 43.80 0.950
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1304.2 7
0010-0043
16.80 44.41 162 53.70 0.706
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 581.6 7
0011-0040
381.00 39.87 117 42.87 0.800
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2014.0 7
0012-0047
30.32 48.28 217 59.94 0.759
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 417.6 7
0013-0047
435.38 48.08 253 60.53 0.868
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 269.3 7
0014-0046
920.57 45.84 220 55.11 0.910
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 298.4 7
0015-0051
272.52 52.16 262 67.50 0.723
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 229.8 7
0016-0050
486.01 50.03 449 81.26 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 271.3 7
0017-0057
78.11 57.33 229 56.77 0.893
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 735.3 7
0018-0063
120.28 62.76 458 79.01 0.922
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 412.5 7
0019-0063
57.27 62.60 233 62.77 0.743