Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 924.9 6
0073-0131
 813.04 132.02 186 52.87 0.836
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 245.6 6
0074-0133
 355.82 133.13 343 67.94 0.934
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 829.8 6
0075-0138
 732.48 138.25 276 61.36 0.921
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 189.7 6
0076-0144
 149.47 145.45 274 73.60 0.636
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1315.3 6
0077-0140
 309.01 139.24 216 55.94 0.867
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1486.8 6
0078-0143
 196.39 143.48 203 56.28 0.805
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1241.4 6
0079-0149
 786.60 150.31 291 65.36 0.856
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 277.8 6
0080-0150
 386.37 149.85 275 60.77 0.936
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1439.8 6
0081-0148
 817.30 148.22 250 75.01 0.558
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 8130.8 6
0082-0150
 946.96 149.91 242 58.18 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1781.5 6
0083-0153
 273.24 154.02 289 65.01 0.859
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 858.3 6
0084-0155
 131.09 155.52 247 59.01 0.891
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 583.9 6
0085-0157
 246.98 157.73 337 69.60 0.874
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 381.1 6
0086-0158
 457.24 158.53 339 68.43 0.910
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 635.6 6
0087-0158
 208.77 158.21 242 58.18 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 901.6 6
0088-0166
 49.73 166.25 354 70.77 0.888
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1440.8 6
0089-0166
 287.54 166.76 248 58.77 0.902
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 264.6 6
0090-0175
 870.84 175.38 381 75.26 0.845
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 855.1 6
0091-0172
 735.60 172.28 234 59.36 0.835
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 217.8 6
0092-0175
 835.95 175.10 250 57.94 0.936
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2890.9 6
0093-0179
 464.94 179.35 215 56.28 0.853
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 305.9 6
0094-0179
 190.45 179.29 343 71.36 0.847
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 11251.2 6
0095-0182
 76.79 183.02 272 67.01 0.761
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 553.9 6
0096-0182
 373.99 181.65 227 79.98 0.446
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1698.8 6
0097-0181
 690.04 180.83 277 62.18 0.900