Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 246.9 5
0298-0530
 464.02 530.85 276 63.94 0.848
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 423.7 5
0299-0535
 526.23 535.26 278 62.53 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 213.5 5
0300-0534
 194.17 534.36 146 49.46 0.750
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 199.5 5
0301-0537
 631.59 536.87 273 62.77 0.871
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 206.2 5
0302-0538
 203.78 538.71 112 38.39 0.955
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 168.4 5
0303-0541
 943.14 542.26 251 61.60 0.831
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 292.7 5
0304-0544
 285.41 544.89 227 58.77 0.826
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3148.7 5
0305-0542
 66.73 542.40 222 54.53 0.938
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1196.3 5
0306-0545
 394.84 545.45 237 57.94 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 280.1 5
0307-0547
 416.32 546.55 299 65.60 0.873
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 200.2 5
0308-0549
 501.07 550.03 257 67.01 0.719
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 258.5 5
0309-0548
 914.84 547.20 322 67.60 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 824.9 5
0310-0547
 861.68 547.45 240 58.18 0.891
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 731.0 5
0311-0550
 333.81 549.73 238 60.43 0.819
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 408.8 5
0312-0552
 96.40 551.94 339 71.26 0.839
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 487.0 5
0313-0550
 271.40 550.14 145 47.70 0.801
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.3 5
0314-0558
 159.75 557.98 250 57.94 0.936
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1789.9 5
0315-0557
 32.26 556.67 214 53.70 0.933
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1281.8 5
0316-0560
 226.45 559.89 257 66.77 0.724
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 614.1 5
0317-0563
 884.68 563.30 252 60.53 0.864
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1405.7 5
0318-0563
 187.93 563.47 259 60.18 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 207.2 5
0319-0566
 488.24 566.76 425 80.43 0.826
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1569.5 5
0320-0567
 726.77 566.37 288 65.01 0.856
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 494.7 5
0321-0570
 782.32 570.74 264 60.53 0.906
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 583.8 5
0322-0572
 138.28 572.28 266 60.53 0.912