Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4153.7 4
0326-0649
 751.38 649.17 248 62.53 0.797
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 218.7 4
0327-0655
 677.54 656.14 307 64.18 0.936
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2753.8 4
0328-0657
 92.49 656.86 234 56.28 0.928
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 243.5 4
0329-0665
 419.08 665.94 283 62.53 0.910
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 345.7 4
0330-0665
 464.09 665.02 258 60.77 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 309.6 4
0331-0668
 138.91 668.35 265 62.18 0.861
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3427.7 4
0332-0670
 579.98 670.48 218 55.70 0.883
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 553.8 4
0333-0669
 157.98 669.52 183 52.53 0.833
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 249.5 4
0334-0677
 304.96 677.31 383 75.01 0.855
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 388.0 4
0335-0676
 369.06 676.55 337 71.84 0.821
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 765.0 4
0336-0676
 106.14 676.43 226 57.11 0.871
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 226.1 4
0337-0681
 489.96 681.89 293 70.53 0.740
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 749.0 4
0338-0679
 219.54 679.54 244 57.94 0.913
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2986.9 4
0339-0682
 439.59 681.45 316 65.60 0.923
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 553.3 4
0340-0682
 341.94 682.00 147 49.11 0.766
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 338.8 4
0341-0686
 771.81 686.37 246 59.36 0.877
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 547.9 4
0342-0687
 352.74 686.86 160 48.87 0.842
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.5 4
0343-0689
 66.90 688.40 228 58.28 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.0 4
0344-0695
 584.28 694.25 440 78.43 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 9220.7 4
0345-0693
 190.14 693.68 257 58.77 0.935
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 463.4 4
0346-0694
 508.55 695.05 278 66.18 0.798
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1512.1 4
0347-0699
 91.54 698.54 186 51.36 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 164.6 4
0348-0701
 321.09 700.57 259 61.94 0.848
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 9308.0 4
0349-0701
 852.08 700.68 243 63.01 0.769
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1259.5 4
0350-0704
 139.10 703.95 232 56.18 0.924