Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 362.35 | p-Rb(S807/S811) | 212.69 | p-ERK(T202/Y204) | 226.97 | 1 | LOXIMVI-signal1 | F11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 300.67 | p-Rb(S807/S811) | 162.97 | p-S6(S235/S236 | 433.52 | 2 | Colo858-signal2 | G10 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 307.03 | p-Rb(S807/S811) | 198.87 | p-ERK(T202/Y204) | 175.67 | 1 | LOXIMVI-signal1 | F10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 311.80 | p-Rb(S807/S811) | 160.43 | p-S6(S235/S236 | 378.06 | 1 | Colo858-signal2 | F9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 337.75 | p-Rb(S807/S811) | 156.22 | p-S6(S235/S236 | 357.37 | 1 | Colo858-signal2 | F8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 219.70 | p-Rb(S807/S811) | 143.66 | p-S6(S235/S236 | 371.59 | 1 | Colo858-signal2 | F7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1676.60 | p-Rb(S807/S811) | 60.57 | p-ERK(T202/Y204) | 20.44 | 2 | Colo858-signal1 | G3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 191.90 | p-Rb(S807/S811) | 81.85 | p-S6(S235/S236 | 199.11 | 1 | Colo858-signal2 | F6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 155.41 | p-Rb(S807/S811) | 59.98 | p-S6(S235/S236 | 99.31 | 1 | Colo858-signal2 | F5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1526.80 | p-Rb(S807/S811) | 101.09 | p-ERK(T202/Y204) | 29.24 | 2 | Colo858-signal1 | G2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 168.97 | p-Rb(S807/S811) | 57.67 | p-S6(S235/S236 | 47.25 | 1 | Colo858-signal2 | F4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 256.94 | p-Rb(S807/S811) | 69.35 | p-S6(S235/S236 | 26.20 | 1 | Colo858-signal2 | F3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 447.88 | p-Rb(S807/S811) | 161.30 | p-ERK(T202/Y204) | 568.39 | 2 | Colo858-signal1 | G11 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 416.80 | p-Rb(S807/S811) | 107.61 | p-S6(S235/S236 | 19.41 | 1 | Colo858-signal2 | F2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 336.86 | p-Rb(S807/S811) | 161.69 | p-S6(S235/S236 | 384.38 | 1 | Colo858-signal2 | F11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 421.06 | p-Rb(S807/S811) | 154.67 | p-ERK(T202/Y204) | 537.34 | 2 | Colo858-signal1 | G10 |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 317.72 | p-Rb(S807/S811) | 203.25 | p-ERK(T202/Y204) | 167.85 | 2 | LOXIMVI-signal1 | C9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 288.89 | p-Rb(S807/S811) | 152.75 | p-S6(S235/S236 | 379.48 | 1 | Colo858-signal2 | F10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.45 | p-Rb(S807/S811) | 160.46 | p-ERK(T202/Y204) | 599.65 | 1 | Colo858-signal1 | F9 |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 319.35 | p-Rb(S807/S811) | 195.10 | p-ERK(T202/Y204) | 133.04 | 2 | LOXIMVI-signal1 | C8 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 346.34 | p-Rb(S807/S811) | 184.23 | p-ERK(T202/Y204) | 94.79 | 2 | LOXIMVI-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 364.85 | p-Rb(S807/S811) | 178.03 | p-ERK(T202/Y204) | 76.08 | 2 | LOXIMVI-signal1 | C6 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 384.63 | p-Rb(S807/S811) | 153.53 | p-ERK(T202/Y204) | 56.47 | 2 | LOXIMVI-signal1 | C5 |