Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.72 | p-Rb(S807/S811) | 84.06 | p-S6(S235/S236 | 180.02 | 2 | LOXIMVI-signal2 | C3 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 237.39 | p-Rb(S807/S811) | 127.65 | p-S6(S235/S236 | 339.13 | 1 | LOXIMVI-signal2 | B4 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 203.52 | p-Rb(S807/S811) | 97.55 | p-S6(S235/S236 | 181.29 | 1 | LOXIMVI-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.99 | p-Rb(S807/S811) | 80.53 | p-S6(S235/S236 | 102.92 | 1 | LOXIMVI-signal2 | B2 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2154.80 | p-Rb(S807/S811) | 145.55 | p-cJun(S73) | 28.64 | 1 | Colo858-signal3 | B10 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 290.41 | p-Rb(S807/S811) | 222.56 | p-S6(S235/S236 | 435.26 | 1 | LOXIMVI-signal2 | B10 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.93 | p-Rb(S807/S811) | 203.95 | p-ERK(T202/Y204) | 169.30 | 2 | LOXIMVI-signal1 | G9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 343.94 | p-Rb(S807/S811) | 170.97 | p-S6(S235/S236 | 450.82 | 2 | Colo858-signal2 | G9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 456.27 | p-Rb(S807/S811) | 159.28 | p-ERK(T202/Y204) | 556.01 | 2 | Colo858-signal1 | G9 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 317.63 | p-Rb(S807/S811) | 191.25 | p-ERK(T202/Y204) | 128.24 | 2 | LOXIMVI-signal1 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 327.85 | p-Rb(S807/S811) | 162.48 | p-S6(S235/S236 | 410.06 | 2 | Colo858-signal2 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 432.08 | p-Rb(S807/S811) | 123.48 | p-ERK(T202/Y204) | 43.03 | 2 | LOXIMVI-signal1 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 239.08 | p-Rb(S807/S811) | 140.42 | p-S6(S235/S236 | 380.85 | 2 | Colo858-signal2 | G7 |