Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 242.80 | p-Rb(S807/S811) | 108.49 | p-ERK(T202/Y204) | 267.00 | 2 | WM1552C-signal1 | E7 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 237.82 | p-Rb(S807/S811) | 112.04 | p-S6(S235/S236 | 394.15 | 1 | WM1552C-signal2 | D10 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 234.24 | p-Rb(S807/S811) | 115.31 | p-S6(S235/S236 | 377.79 | 1 | WM1552C-signal2 | D11 | |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 226.81 | p-Rb(S807/S811) | 116.31 | p-S6(S235/S236 | 435.53 | 1 | WM1552C-signal2 | D3 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 262.28 | p-Rb(S807/S811) | 113.37 | p-S6(S235/S236 | 413.92 | 1 | WM1552C-signal2 | D4 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 439.43 | p-Rb(S807/S811) | 169.34 | p-ERK(T202/Y204) | 657.25 | 1 | Colo858-signal1 | D8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 244.72 | p-Rb(S807/S811) | 115.35 | p-S6(S235/S236 | 425.96 | 1 | WM1552C-signal2 | D8 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 192.93 | p-Rb(S807/S811) | 112.08 | p-S6(S235/S236 | 409.76 | 2 | WM1552C-signal2 | E10 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 200.92 | p-Rb(S807/S811) | 113.95 | p-S6(S235/S236 | 415.95 | 2 | WM1552C-signal2 | E11 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 204.00 | p-Rb(S807/S811) | 111.59 | p-S6(S235/S236 | 385.25 | 2 | WM1552C-signal2 | E4 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 238.67 | p-Rb(S807/S811) | 112.35 | p-S6(S235/S236 | 403.49 | 2 | WM1552C-signal2 | E5 | |
| JNK-IN-8 | WM1552C | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 202.01 | p-Rb(S807/S811) | 113.23 | p-S6(S235/S236 | 403.64 | 2 | WM1552C-signal2 | E7 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 205.45 | p-Rb(S807/S811) | 114.75 | p-S6(S235/S236 | 404.26 | 2 | WM1552C-signal2 | E8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | Ki-67 | 1543.30 | p-Rb(S807/S811) | 112.25 | p-cJun(S73) | 28.35 | 1 | WM1552C-signal3 | D3 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 298.38 | p-Rb(S807/S811) | 219.79 | p-S6(S235/S236 | 438.42 | 1 | LOXIMVI-signal2 | B8 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 255.47 | p-Rb(S807/S811) | 192.91 | p-S6(S235/S236 | 440.25 | 1 | LOXIMVI-signal2 | B7 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 772.11 | p-Rb(S807/S811) | 44.73 | p-cJun(S73) | 66.84 | 1 | Colo858-signal3 | B3 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 |