Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2947.60 | p-Rb(S807/S811) | 217.27 | p-cJun(S73) | 73.06 | 1 | LOXIMVI-signal3 | D7 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | Ki-67 | 2963.80 | p-Rb(S807/S811) | 214.47 | p-cJun(S73) | 101.55 | 1 | LOXIMVI-signal3 | D9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | Ki-67 | 2882.90 | p-Rb(S807/S811) | 203.40 | p-cJun(S73) | 97.65 | 1 | LOXIMVI-signal3 | F10 |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 341.90 | p-Rb(S807/S811) | 169.94 | p-S6(S235/S236 | 414.55 | 1 | Colo858-signal2 | B9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 269.36 | p-Rb(S807/S811) | 224.35 | p-S6(S235/S236 | 447.46 | 1 | LOXIMVI-signal2 | F10 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.85 | p-Rb(S807/S811) | 104.29 | p-S6(S235/S236 | 19.60 | 1 | LOXIMVI-signal2 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 229.67 | p-Rb(S807/S811) | 98.04 | p-S6(S235/S236 | 97.15 | 1 | LOXIMVI-signal2 | F3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 229.22 | p-Rb(S807/S811) | 100.85 | p-S6(S235/S236 | 225.60 | 1 | LOXIMVI-signal2 | F4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 249.86 | p-Rb(S807/S811) | 181.43 | p-S6(S235/S236 | 413.04 | 1 | LOXIMVI-signal2 | F6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 233.98 | p-Rb(S807/S811) | 191.85 | p-S6(S235/S236 | 428.50 | 1 | LOXIMVI-signal2 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 267.75 | p-Rb(S807/S811) | 208.98 | p-S6(S235/S236 | 429.59 | 1 | LOXIMVI-signal2 | F8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 238.51 | p-Rb(S807/S811) | 220.71 | p-S6(S235/S236 | 444.78 | 1 | LOXIMVI-signal2 | F9 |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 2383.20 | p-Rb(S807/S811) | 179.73 | p-cJun(S73) | 31.88 | 1 | Colo858-signal3 | D4 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 2279.00 | p-Rb(S807/S811) | 170.95 | p-cJun(S73) | 30.51 | 1 | Colo858-signal3 | D5 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 2238.80 | p-Rb(S807/S811) | 159.49 | p-cJun(S73) | 30.74 | 1 | Colo858-signal3 | D6 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 325.41 | p-Rb(S807/S811) | 43.62 | p-ERK(T202/Y204) | 15.11 | 1 | WM115-signal1 | B3 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 2200.30 | p-Rb(S807/S811) | 153.90 | p-cJun(S73) | 30.11 | 1 | Colo858-signal3 | D7 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.09 | p-Rb(S807/S811) | 68.88 | p-ERK(T202/Y204) | 366.99 | 1 | WM115-signal1 | B7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2267.30 | p-Rb(S807/S811) | 156.38 | p-cJun(S73) | 31.07 | 1 | Colo858-signal3 | D8 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 230.43 | p-Rb(S807/S811) | 73.12 | p-ERK(T202/Y204) | 557.15 | 1 | WM115-signal1 | B9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2226.10 | p-Rb(S807/S811) | 150.62 | p-cJun(S73) | 29.95 | 1 | Colo858-signal3 | D9 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 256.65 | p-Rb(S807/S811) | 104.44 | p-ERK(T202/Y204) | 246.25 | 1 | WM1552C-signal1 | F9 |