Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 863.33 | p-Rb(S807/S811) | 76.07 | p-cJun(S73) | 41.68 | 1 | WM115-signal3 | F7 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 1742.30 | p-Rb(S807/S811) | 126.75 | p-cJun(S73) | 38.16 | 1 | Colo858-signal3 | B7 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 163.91 | p-Rb(S807/S811) | 54.08 | p-S6(S235/S236 | 23.01 | 1 | Colo858-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2103.40 | p-Rb(S807/S811) | 149.11 | p-cJun(S73) | 30.17 | 1 | Colo858-signal3 | B8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2239.40 | p-Rb(S807/S811) | 151.71 | p-cJun(S73) | 29.36 | 1 | Colo858-signal3 | B9 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 283.24 | p-Rb(S807/S811) | 237.66 | p-S6(S235/S236 | 456.10 | 1 | LOXIMVI-signal2 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 299.94 | p-Rb(S807/S811) | 232.62 | p-S6(S235/S236 | 413.74 | 1 | LOXIMVI-signal2 | D11 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 388.24 | p-Rb(S807/S811) | 277.19 | p-S6(S235/S236 | 582.84 | 1 | LOXIMVI-signal2 | D2 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 368.60 | p-Rb(S807/S811) | 272.58 | p-S6(S235/S236 | 627.74 | 1 | LOXIMVI-signal2 | D3 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 344.33 | p-Rb(S807/S811) | 248.42 | p-S6(S235/S236 | 524.11 | 1 | LOXIMVI-signal2 | D4 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 290.00 | p-Rb(S807/S811) | 244.26 | p-S6(S235/S236 | 469.02 | 1 | LOXIMVI-signal2 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 285.05 | p-Rb(S807/S811) | 232.52 | p-S6(S235/S236 | 459.23 | 1 | LOXIMVI-signal2 | D7 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 267.83 | p-Rb(S807/S811) | 225.75 | p-S6(S235/S236 | 426.66 | 1 | LOXIMVI-signal2 | D9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 233.98 | p-Rb(S807/S811) | 191.85 | p-S6(S235/S236 | 428.50 | 1 | LOXIMVI-signal2 | F7 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2047.60 | p-Rb(S807/S811) | 78.14 | p-cJun(S73) | 80.02 | 1 | LOXIMVI-signal3 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1016.50 | p-Rb(S807/S811) | 42.64 | p-ERK(T202/Y204) | 14.04 | 1 | Colo858-signal1 | B3 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2357.40 | p-Rb(S807/S811) | 128.12 | p-cJun(S73) | 82.54 | 1 | LOXIMVI-signal3 | B4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 442.40 | p-Rb(S807/S811) | 51.64 | p-ERK(T202/Y204) | 26.95 | 1 | WM1552C-signal1 | B4 |