Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 237.39 | p-Rb(S807/S811) | 127.65 | p-S6(S235/S236 | 339.13 | 1 | LOXIMVI-signal2 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1063.20 | p-Rb(S807/S811) | 48.29 | p-ERK(T202/Y204) | 17.48 | 1 | Colo858-signal1 | B4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 153.80 | p-Rb(S807/S811) | 53.66 | p-S6(S235/S236 | 392.09 | 1 | WM1552C-signal2 | B4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | Ki-67 | 1044.70 | p-Rb(S807/S811) | 52.94 | p-cJun(S73) | 128.12 | 1 | WM1552C-signal3 | B4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 442.40 | p-Rb(S807/S811) | 51.64 | p-ERK(T202/Y204) | 26.95 | 1 | WM1552C-signal1 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 663.63 | p-Rb(S807/S811) | 39.63 | p-cJun(S73) | 79.94 | 1 | WM115-signal3 | B4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 437.65 | p-Rb(S807/S811) | 139.21 | p-ERK(T202/Y204) | 46.29 | 1 | LOXIMVI-signal1 | B4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 190.48 | p-Rb(S807/S811) | 38.37 | p-S6(S235/S236 | 66.01 | 1 | WM115-signal2 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 165.33 | p-Rb(S807/S811) | 58.60 | p-S6(S235/S236 | 48.12 | 1 | Colo858-signal2 | B4 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2470.00 | p-Rb(S807/S811) | 138.22 | p-cJun(S73) | 75.91 | 1 | LOXIMVI-signal3 | B5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 395.79 | p-Rb(S807/S811) | 157.15 | p-ERK(T202/Y204) | 59.04 | 1 | LOXIMVI-signal1 | B5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 964.47 | p-Rb(S807/S811) | 50.08 | p-ERK(T202/Y204) | 25.09 | 1 | Colo858-signal1 | B5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 263.18 | p-Rb(S807/S811) | 37.97 | p-ERK(T202/Y204) | 43.14 | 1 | WM115-signal1 | B5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 180.39 | p-Rb(S807/S811) | 38.47 | p-S6(S235/S236 | 77.58 | 1 | WM115-signal2 | B5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | Ki-67 | 1190.60 | p-Rb(S807/S811) | 70.53 | p-cJun(S73) | 155.05 | 1 | WM1552C-signal3 | B5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.13 | p-Rb(S807/S811) | 66.51 | p-S6(S235/S236 | 373.65 | 1 | WM1552C-signal2 | B5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 337.86 | p-Rb(S807/S811) | 61.82 | p-ERK(T202/Y204) | 50.97 | 1 | WM1552C-signal1 | B5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 721.37 | p-Rb(S807/S811) | 38.52 | p-cJun(S73) | 78.48 | 1 | WM115-signal3 | B5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.45 | p-Rb(S807/S811) | 54.34 | p-S6(S235/S236 | 77.03 | 1 | Colo858-signal2 | B5 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 784.48 | p-Rb(S807/S811) | 55.88 | p-cJun(S73) | 67.92 | 1 | WM115-signal3 | B6 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 366.51 | p-Rb(S807/S811) | 180.61 | p-ERK(T202/Y204) | 84.29 | 1 | LOXIMVI-signal1 | B6 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 |