Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 336.86 | p-Rb(S807/S811) | 161.69 | p-S6(S235/S236 | 384.38 | 1 | Colo858-signal2 | F11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2257.00 | p-Rb(S807/S811) | 151.09 | p-cJun(S73) | 29.94 | 1 | Colo858-signal3 | F11 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 3032.30 | p-Rb(S807/S811) | 209.50 | p-cJun(S73) | 127.94 | 1 | LOXIMVI-signal3 | F11 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 239.37 | p-Rb(S807/S811) | 107.35 | p-ERK(T202/Y204) | 253.29 | 1 | WM1552C-signal1 | F11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1646.10 | p-Rb(S807/S811) | 108.76 | p-ERK(T202/Y204) | 29.73 | 1 | Colo858-signal1 | F2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.10 | p-Rb(S807/S811) | 64.53 | p-S6(S235/S236 | 16.11 | 1 | WM1552C-signal2 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2814.00 | p-Rb(S807/S811) | 96.96 | p-cJun(S73) | 30.63 | 1 | LOXIMVI-signal3 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 598.80 | p-Rb(S807/S811) | 103.77 | p-ERK(T202/Y204) | 63.79 | 1 | LOXIMVI-signal1 | F2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1967.00 | p-Rb(S807/S811) | 99.41 | p-cJun(S73) | 22.51 | 1 | Colo858-signal3 | F2 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 274.41 | p-Rb(S807/S811) | 61.27 | p-S6(S235/S236 | 22.13 | 1 | WM115-signal2 | F2 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 614.46 | p-Rb(S807/S811) | 62.48 | p-cJun(S73) | 20.25 | 1 | WM115-signal3 | F2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1057.80 | p-Rb(S807/S811) | 58.43 | p-cJun(S73) | 14.63 | 1 | WM1552C-signal3 | F2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 397.69 | p-Rb(S807/S811) | 59.60 | p-ERK(T202/Y204) | 26.88 | 1 | WM1552C-signal1 | F2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 416.80 | p-Rb(S807/S811) | 107.61 | p-S6(S235/S236 | 19.41 | 1 | Colo858-signal2 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.85 | p-Rb(S807/S811) | 104.29 | p-S6(S235/S236 | 19.60 | 1 | LOXIMVI-signal2 | F2 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 349.44 | p-Rb(S807/S811) | 64.09 | p-ERK(T202/Y204) | 21.49 | 1 | WM115-signal1 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 229.67 | p-Rb(S807/S811) | 98.04 | p-S6(S235/S236 | 97.15 | 1 | LOXIMVI-signal2 | F3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 1000.20 | p-Rb(S807/S811) | 47.24 | p-cJun(S73) | 12.90 | 1 | WM1552C-signal3 | F3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 261.27 | p-Rb(S807/S811) | 47.45 | p-S6(S235/S236 | 41.16 | 1 | WM115-signal2 | F3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 161.46 | p-Rb(S807/S811) | 50.96 | p-S6(S235/S236 | 229.99 | 1 | WM1552C-signal2 | F3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 560.57 | p-Rb(S807/S811) | 82.73 | p-ERK(T202/Y204) | 44.60 | 1 | LOXIMVI-signal1 | F3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 256.94 | p-Rb(S807/S811) | 69.35 | p-S6(S235/S236 | 26.20 | 1 | Colo858-signal2 | F3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 2379.60 | p-Rb(S807/S811) | 93.92 | p-cJun(S73) | 26.61 | 1 | LOXIMVI-signal3 | F3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 337.11 | p-Rb(S807/S811) | 45.84 | p-ERK(T202/Y204) | 20.19 | 1 | WM115-signal1 | F3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 478.70 | p-Rb(S807/S811) | 48.18 | p-ERK(T202/Y204) | 22.45 | 1 | WM1552C-signal1 | F3 |