Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 973.61 | p-Rb(S807/S811) | 47.01 | p-cJun(S73) | 59.51 | 1 | WM1552C-signal3 | B2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 333.89 | p-Rb(S807/S811) | 50.05 | p-ERK(T202/Y204) | 12.54 | 1 | WM115-signal1 | B2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2047.60 | p-Rb(S807/S811) | 78.14 | p-cJun(S73) | 80.02 | 1 | LOXIMVI-signal3 | B2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 691.81 | p-Rb(S807/S811) | 49.35 | p-cJun(S73) | 49.25 | 1 | WM115-signal3 | B2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.89 | p-Rb(S807/S811) | 45.77 | p-S6(S235/S236 | 35.99 | 1 | WM115-signal2 | B2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 499.44 | p-Rb(S807/S811) | 81.11 | p-ERK(T202/Y204) | 31.87 | 1 | LOXIMVI-signal1 | B2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 174.85 | p-Rb(S807/S811) | 52.10 | p-S6(S235/S236 | 261.40 | 1 | WM1552C-signal2 | B2 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 227.79 | p-Rb(S807/S811) | 42.29 | p-S6(S235/S236 | 56.17 | 1 | WM115-signal2 | B3 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 2059.50 | p-Rb(S807/S811) | 90.66 | p-cJun(S73) | 86.47 | 1 | LOXIMVI-signal3 | B3 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 772.11 | p-Rb(S807/S811) | 44.73 | p-cJun(S73) | 66.84 | 1 | Colo858-signal3 | B3 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | Ki-67 | 913.80 | p-Rb(S807/S811) | 42.09 | p-cJun(S73) | 98.86 | 1 | WM1552C-signal3 | B3 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 473.39 | p-Rb(S807/S811) | 42.61 | p-ERK(T202/Y204) | 22.62 | 1 | WM1552C-signal1 | B3 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 123.39 | p-Rb(S807/S811) | 42.15 | p-S6(S235/S236 | 347.41 | 1 | WM1552C-signal2 | B3 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 163.91 | p-Rb(S807/S811) | 54.08 | p-S6(S235/S236 | 23.01 | 1 | Colo858-signal2 | B3 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1016.50 | p-Rb(S807/S811) | 42.64 | p-ERK(T202/Y204) | 14.04 | 1 | Colo858-signal1 | B3 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 456.75 | p-Rb(S807/S811) | 94.75 | p-ERK(T202/Y204) | 28.87 | 1 | LOXIMVI-signal1 | B3 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 671.20 | p-Rb(S807/S811) | 44.63 | p-cJun(S73) | 68.10 | 1 | WM115-signal3 | B3 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 325.41 | p-Rb(S807/S811) | 43.62 | p-ERK(T202/Y204) | 15.11 | 1 | WM115-signal1 | B3 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 203.52 | p-Rb(S807/S811) | 97.55 | p-S6(S235/S236 | 181.29 | 1 | LOXIMVI-signal2 | B3 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 304.70 | p-Rb(S807/S811) | 38.57 | p-ERK(T202/Y204) | 23.27 | 1 | WM115-signal1 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1063.20 | p-Rb(S807/S811) | 48.29 | p-ERK(T202/Y204) | 17.48 | 1 | Colo858-signal1 | B4 |