Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 236.84 | p-Rb(S807/S811) | 83.75 | p-ERK(T202/Y204) | 640.13 | 1 | WM115-signal1 | D5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 453.72 | p-Rb(S807/S811) | 173.99 | p-ERK(T202/Y204) | 686.67 | 1 | Colo858-signal1 | D5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 205.29 | p-Rb(S807/S811) | 76.65 | p-S6(S235/S236 | 177.08 | 1 | WM115-signal2 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 312.28 | p-Rb(S807/S811) | 224.98 | p-ERK(T202/Y204) | 217.75 | 1 | LOXIMVI-signal1 | D5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 235.99 | p-Rb(S807/S811) | 105.87 | p-ERK(T202/Y204) | 263.93 | 1 | WM1552C-signal1 | D5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 367.65 | p-Rb(S807/S811) | 192.54 | p-S6(S235/S236 | 440.93 | 1 | Colo858-signal2 | D5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | Ki-67 | 1583.90 | p-Rb(S807/S811) | 110.94 | p-cJun(S73) | 105.82 | 1 | WM1552C-signal3 | D5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 249.08 | p-Rb(S807/S811) | 114.01 | p-S6(S235/S236 | 401.71 | 1 | WM1552C-signal2 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2939.70 | p-Rb(S807/S811) | 217.05 | p-cJun(S73) | 57.83 | 1 | LOXIMVI-signal3 | D5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 2279.00 | p-Rb(S807/S811) | 170.95 | p-cJun(S73) | 30.51 | 1 | Colo858-signal3 | D5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 814.81 | p-Rb(S807/S811) | 81.21 | p-cJun(S73) | 22.95 | 1 | WM115-signal3 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 450.18 | p-Rb(S807/S811) | 173.59 | p-ERK(T202/Y204) | 691.16 | 1 | Colo858-signal1 | D6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 250.36 | p-Rb(S807/S811) | 85.23 | p-ERK(T202/Y204) | 658.54 | 1 | WM115-signal1 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 319.20 | p-Rb(S807/S811) | 216.66 | p-ERK(T202/Y204) | 220.23 | 1 | LOXIMVI-signal1 | D6 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 241.67 | p-Rb(S807/S811) | 111.54 | p-ERK(T202/Y204) | 273.53 | 1 | WM1552C-signal1 | D6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 843.01 | p-Rb(S807/S811) | 77.92 | p-cJun(S73) | 26.33 | 1 | WM115-signal3 | D6 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | Ki-67 | 1599.10 | p-Rb(S807/S811) | 106.57 | p-cJun(S73) | 132.61 | 1 | WM1552C-signal3 | D6 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 319.80 | p-Rb(S807/S811) | 180.35 | p-S6(S235/S236 | 422.77 | 1 | Colo858-signal2 | D6 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 221.29 | p-Rb(S807/S811) | 114.21 | p-S6(S235/S236 | 384.84 | 1 | WM1552C-signal2 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2922.80 | p-Rb(S807/S811) | 202.58 | p-cJun(S73) | 68.33 | 1 | LOXIMVI-signal3 | D6 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 2238.80 | p-Rb(S807/S811) | 159.49 | p-cJun(S73) | 30.74 | 1 | Colo858-signal3 | D6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 238.95 | p-Rb(S807/S811) | 79.96 | p-S6(S235/S236 | 176.83 | 1 | WM115-signal2 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 290.00 | p-Rb(S807/S811) | 244.26 | p-S6(S235/S236 | 469.02 | 1 | LOXIMVI-signal2 | D6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 |