Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 223.26 | p-Rb(S807/S811) | 102.60 | p-S6(S235/S236 | 381.13 | 2 | WM1552C-signal2 | C8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2138.80 | p-Rb(S807/S811) | 144.45 | p-cJun(S73) | 30.43 | 2 | Colo858-signal3 | C8 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | Ki-67 | 1538.30 | p-Rb(S807/S811) | 102.40 | p-cJun(S73) | 142.93 | 2 | WM1552C-signal3 | C9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 229.99 | p-Rb(S807/S811) | 110.00 | p-S6(S235/S236 | 389.09 | 2 | WM1552C-signal2 | C9 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | Ki-67 | 2983.70 | p-Rb(S807/S811) | 203.05 | p-cJun(S73) | 109.97 | 2 | LOXIMVI-signal3 | C9 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | Ki-67 | 730.04 | p-Rb(S807/S811) | 70.31 | p-cJun(S73) | 48.98 | 2 | WM115-signal3 | C9 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 248.41 | p-Rb(S807/S811) | 70.27 | p-S6(S235/S236 | 185.43 | 2 | WM115-signal2 | C9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 317.72 | p-Rb(S807/S811) | 203.25 | p-ERK(T202/Y204) | 167.85 | 2 | LOXIMVI-signal1 | C9 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.92 | p-Rb(S807/S811) | 163.29 | p-ERK(T202/Y204) | 613.35 | 2 | Colo858-signal1 | C9 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 264.06 | p-Rb(S807/S811) | 220.85 | p-S6(S235/S236 | 434.18 | 2 | LOXIMVI-signal2 | C9 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 301.75 | p-Rb(S807/S811) | 164.37 | p-S6(S235/S236 | 394.95 | 2 | Colo858-signal2 | C9 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 224.35 | p-Rb(S807/S811) | 70.42 | p-ERK(T202/Y204) | 575.35 | 2 | WM115-signal1 | C9 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2183.20 | p-Rb(S807/S811) | 146.71 | p-cJun(S73) | 28.18 | 2 | Colo858-signal3 | C9 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 237.82 | p-Rb(S807/S811) | 112.04 | p-S6(S235/S236 | 394.15 | 1 | WM1552C-signal2 | D10 | |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 197.00 | p-Rb(S807/S811) | 65.37 | p-S6(S235/S236 | 186.60 | 1 | WM115-signal2 | D10 | |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | Ki-67 | 783.34 | p-Rb(S807/S811) | 75.14 | p-cJun(S73) | 46.08 | 1 | WM115-signal3 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | Ki-67 | 2844.80 | p-Rb(S807/S811) | 201.31 | p-cJun(S73) | 107.70 | 1 | LOXIMVI-signal3 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 307.47 | p-Rb(S807/S811) | 207.98 | p-ERK(T202/Y204) | 211.16 | 1 | LOXIMVI-signal1 | D10 | |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 227.05 | p-Rb(S807/S811) | 74.53 | p-ERK(T202/Y204) | 625.81 | 1 | WM115-signal1 | D10 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 237.48 | p-Rb(S807/S811) | 106.12 | p-ERK(T202/Y204) | 259.42 | 1 | WM1552C-signal1 | D10 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 438.30 | p-Rb(S807/S811) | 160.10 | p-ERK(T202/Y204) | 626.02 | 1 | Colo858-signal1 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 283.24 | p-Rb(S807/S811) | 237.66 | p-S6(S235/S236 | 456.10 | 1 | LOXIMVI-signal2 | D10 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 280.73 | p-Rb(S807/S811) | 169.28 | p-S6(S235/S236 | 409.65 | 1 | Colo858-signal2 | D10 |