Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 274.60 | p-Rb(S807/S811) | 140.30 | p-S6(S235/S236 | 340.20 | 2 | Colo858-signal2 | C7 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 204.23 | p-Rb(S807/S811) | 70.93 | p-S6(S235/S236 | 342.80 | 1 | WM1552C-signal2 | F5 |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 123.39 | p-Rb(S807/S811) | 42.15 | p-S6(S235/S236 | 347.41 | 1 | WM1552C-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 167.82 | p-Rb(S807/S811) | 73.52 | p-S6(S235/S236 | 355.93 | 2 | WM1552C-signal2 | C5 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 177.12 | p-Rb(S807/S811) | 56.01 | p-S6(S235/S236 | 356.27 | 2 | WM1552C-signal2 | C4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 337.75 | p-Rb(S807/S811) | 156.22 | p-S6(S235/S236 | 357.37 | 1 | Colo858-signal2 | F8 |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 230.57 | p-Rb(S807/S811) | 92.72 | p-S6(S235/S236 | 359.12 | 2 | WM1552C-signal2 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 165.37 | p-Rb(S807/S811) | 90.82 | p-S6(S235/S236 | 359.54 | 1 | WM1552C-signal2 | F6 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 158.30 | p-Rb(S807/S811) | 60.54 | p-S6(S235/S236 | 363.25 | 1 | WM1552C-signal2 | F4 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 235.54 | p-Rb(S807/S811) | 72.02 | p-ERK(T202/Y204) | 366.04 | 2 | WM115-signal1 | G7 |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.09 | p-Rb(S807/S811) | 68.88 | p-ERK(T202/Y204) | 366.99 | 1 | WM115-signal1 | B7 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 219.70 | p-Rb(S807/S811) | 143.66 | p-S6(S235/S236 | 371.59 | 1 | Colo858-signal2 | F7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 214.37 | p-Rb(S807/S811) | 82.91 | p-S6(S235/S236 | 372.87 | 1 | WM1552C-signal2 | F9 |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.13 | p-Rb(S807/S811) | 66.51 | p-S6(S235/S236 | 373.65 | 1 | WM1552C-signal2 | B5 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 224.64 | p-Rb(S807/S811) | 95.99 | p-S6(S235/S236 | 373.81 | 2 | WM1552C-signal2 | C7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 233.71 | p-Rb(S807/S811) | 136.67 | p-S6(S235/S236 | 375.36 | 2 | LOXIMVI-signal2 | G5 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 190.31 | p-Rb(S807/S811) | 96.89 | p-S6(S235/S236 | 375.87 | 1 | WM1552C-signal2 | F7 |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 208.13 | p-Rb(S807/S811) | 96.36 | p-S6(S235/S236 | 376.62 | 1 | WM1552C-signal2 | B6 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 234.24 | p-Rb(S807/S811) | 115.31 | p-S6(S235/S236 | 377.79 | 1 | WM1552C-signal2 | D11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 311.80 | p-Rb(S807/S811) | 160.43 | p-S6(S235/S236 | 378.06 | 1 | Colo858-signal2 | F9 |