Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 472.02 | p-Rb(S807/S811) | 42.22 | p-ERK(T202/Y204) | 19.71 | 2 | WM1552C-signal1 | C3 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 637.76 | p-Rb(S807/S811) | 61.90 | p-cJun(S73) | 19.77 | 2 | WM115-signal3 | G2 |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 790.07 | p-Rb(S807/S811) | 77.44 | p-cJun(S73) | 19.89 | 1 | WM115-signal3 | D3 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 744.48 | p-Rb(S807/S811) | 42.82 | p-cJun(S73) | 20.07 | 1 | WM115-signal3 | F4 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 337.11 | p-Rb(S807/S811) | 45.84 | p-ERK(T202/Y204) | 20.19 | 1 | WM115-signal1 | F3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 614.46 | p-Rb(S807/S811) | 62.48 | p-cJun(S73) | 20.25 | 1 | WM115-signal3 | F2 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 682.82 | p-Rb(S807/S811) | 40.64 | p-cJun(S73) | 20.31 | 2 | WM115-signal3 | G4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1676.60 | p-Rb(S807/S811) | 60.57 | p-ERK(T202/Y204) | 20.44 | 2 | Colo858-signal1 | G3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 797.23 | p-Rb(S807/S811) | 51.78 | p-cJun(S73) | 20.45 | 1 | Colo858-signal3 | F5 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 339.45 | p-Rb(S807/S811) | 47.47 | p-ERK(T202/Y204) | 20.55 | 2 | WM115-signal1 | G3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 347.97 | p-Rb(S807/S811) | 60.14 | p-ERK(T202/Y204) | 20.70 | 2 | WM115-signal1 | G2 |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 812.54 | p-Rb(S807/S811) | 82.01 | p-cJun(S73) | 21.32 | 1 | WM115-signal3 | D4 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 349.44 | p-Rb(S807/S811) | 64.09 | p-ERK(T202/Y204) | 21.49 | 1 | WM115-signal1 | F2 |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 1507.90 | p-Rb(S807/S811) | 107.69 | p-cJun(S73) | 21.50 | 2 | WM1552C-signal3 | E2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 150.06 | p-Rb(S807/S811) | 51.74 | p-S6(S235/S236 | 21.56 | 2 | Colo858-signal2 | C3 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 785.96 | p-Rb(S807/S811) | 71.52 | p-cJun(S73) | 21.74 | 2 | WM115-signal3 | E5 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 800.51 | p-Rb(S807/S811) | 84.77 | p-cJun(S73) | 21.81 | 2 | WM115-signal3 | E4 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 433.95 | p-Rb(S807/S811) | 51.26 | p-ERK(T202/Y204) | 22.13 | 2 | WM1552C-signal1 | C2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 274.41 | p-Rb(S807/S811) | 61.27 | p-S6(S235/S236 | 22.13 | 1 | WM115-signal2 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 351.16 | p-Rb(S807/S811) | 100.40 | p-S6(S235/S236 | 22.18 | 2 | LOXIMVI-signal2 | G2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 756.62 | p-Rb(S807/S811) | 56.61 | p-cJun(S73) | 22.24 | 2 | Colo858-signal3 | G5 |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 815.32 | p-Rb(S807/S811) | 84.33 | p-cJun(S73) | 22.27 | 2 | WM115-signal3 | E3 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1532.60 | p-Rb(S807/S811) | 62.03 | p-ERK(T202/Y204) | 22.35 | 1 | Colo858-signal1 | F3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |