Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2570.00 | p-Rb(S807/S811) | 157.19 | p-cJun(S73) | 85.99 | 2 | LOXIMVI-signal3 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 338.89 | p-Rb(S807/S811) | 176.50 | p-ERK(T202/Y204) | 86.35 | 1 | LOXIMVI-signal1 | F7 |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2653.30 | p-Rb(S807/S811) | 167.23 | p-cJun(S73) | 86.38 | 1 | LOXIMVI-signal3 | B6 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 2059.50 | p-Rb(S807/S811) | 90.66 | p-cJun(S73) | 86.47 | 1 | LOXIMVI-signal3 | B3 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2629.00 | p-Rb(S807/S811) | 170.98 | p-cJun(S73) | 89.13 | 2 | LOXIMVI-signal3 | C7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.93 | p-Rb(S807/S811) | 91.17 | p-S6(S235/S236 | 89.76 | 2 | LOXIMVI-signal2 | G3 |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 3052.30 | p-Rb(S807/S811) | 224.01 | p-cJun(S73) | 90.47 | 1 | LOXIMVI-signal3 | D8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2742.00 | p-Rb(S807/S811) | 188.71 | p-cJun(S73) | 92.00 | 1 | LOXIMVI-signal3 | F9 |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 150.91 | p-Rb(S807/S811) | 54.62 | p-S6(S235/S236 | 93.11 | 2 | Colo858-signal2 | C5 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 344.62 | p-Rb(S807/S811) | 191.81 | p-ERK(T202/Y204) | 94.25 | 1 | LOXIMVI-signal1 | B7 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | Ki-67 | 2876.00 | p-Rb(S807/S811) | 199.31 | p-cJun(S73) | 94.47 | 2 | LOXIMVI-signal3 | E9 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 1190.30 | p-Rb(S807/S811) | 65.76 | p-cJun(S73) | 94.49 | 1 | WM1552C-signal3 | F5 |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 346.34 | p-Rb(S807/S811) | 184.23 | p-ERK(T202/Y204) | 94.79 | 2 | LOXIMVI-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2730.30 | p-Rb(S807/S811) | 177.84 | p-cJun(S73) | 95.29 | 1 | LOXIMVI-signal3 | B7 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 2785.20 | p-Rb(S807/S811) | 185.62 | p-cJun(S73) | 96.25 | 1 | LOXIMVI-signal3 | B8 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 1190.00 | p-Rb(S807/S811) | 68.22 | p-cJun(S73) | 96.63 | 2 | WM1552C-signal3 | G5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 229.67 | p-Rb(S807/S811) | 98.04 | p-S6(S235/S236 | 97.15 | 1 | LOXIMVI-signal2 | F3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | Ki-67 | 2882.90 | p-Rb(S807/S811) | 203.40 | p-cJun(S73) | 97.65 | 1 | LOXIMVI-signal3 | F10 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | Ki-67 | 2829.00 | p-Rb(S807/S811) | 197.37 | p-cJun(S73) | 98.13 | 2 | LOXIMVI-signal3 | G10 |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | Ki-67 | 913.80 | p-Rb(S807/S811) | 42.09 | p-cJun(S73) | 98.86 | 1 | WM1552C-signal3 | B3 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 155.41 | p-Rb(S807/S811) | 59.98 | p-S6(S235/S236 | 99.31 | 1 | Colo858-signal2 | F5 |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | Ki-67 | 885.89 | p-Rb(S807/S811) | 44.19 | p-cJun(S73) | 99.31 | 2 | WM1552C-signal3 | C3 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | Ki-67 | 2963.80 | p-Rb(S807/S811) | 214.47 | p-cJun(S73) | 101.55 | 1 | LOXIMVI-signal3 | D9 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 2858.70 | p-Rb(S807/S811) | 191.23 | p-cJun(S73) | 102.21 | 2 | LOXIMVI-signal3 | C8 |