Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 973.61 | p-Rb(S807/S811) | 47.01 | p-cJun(S73) | 59.51 | 1 | WM1552C-signal3 | B2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 955.89 | p-Rb(S807/S811) | 49.08 | p-cJun(S73) | 59.90 | 2 | WM1552C-signal3 | C2 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 1050.90 | p-Rb(S807/S811) | 82.58 | p-cJun(S73) | 62.66 | 2 | Colo858-signal3 | C6 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 771.14 | p-Rb(S807/S811) | 55.68 | p-cJun(S73) | 62.70 | 2 | WM115-signal3 | C6 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | Ki-67 | 1714.80 | p-Rb(S807/S811) | 114.20 | p-cJun(S73) | 63.58 | 1 | WM1552C-signal3 | D4 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2886.70 | p-Rb(S807/S811) | 203.46 | p-cJun(S73) | 63.66 | 2 | LOXIMVI-signal3 | E6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 598.80 | p-Rb(S807/S811) | 103.77 | p-ERK(T202/Y204) | 63.79 | 1 | LOXIMVI-signal1 | F2 |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 148.64 | p-Rb(S807/S811) | 35.95 | p-S6(S235/S236 | 64.00 | 2 | WM115-signal2 | C5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 349.70 | p-Rb(S807/S811) | 71.79 | p-ERK(T202/Y204) | 64.17 | 2 | WM1552C-signal1 | C5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2734.80 | p-Rb(S807/S811) | 188.75 | p-cJun(S73) | 65.47 | 1 | LOXIMVI-signal3 | F8 |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 179.29 | p-Rb(S807/S811) | 38.89 | p-S6(S235/S236 | 65.75 | 2 | WM115-signal2 | C4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 190.48 | p-Rb(S807/S811) | 38.37 | p-S6(S235/S236 | 66.01 | 1 | WM115-signal2 | B4 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 772.11 | p-Rb(S807/S811) | 44.73 | p-cJun(S73) | 66.84 | 1 | Colo858-signal3 | B3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 784.48 | p-Rb(S807/S811) | 55.88 | p-cJun(S73) | 67.92 | 1 | WM115-signal3 | B6 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 660.32 | p-Rb(S807/S811) | 48.67 | p-cJun(S73) | 67.95 | 2 | Colo858-signal3 | C5 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 671.20 | p-Rb(S807/S811) | 44.63 | p-cJun(S73) | 68.10 | 1 | WM115-signal3 | B3 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2922.80 | p-Rb(S807/S811) | 202.58 | p-cJun(S73) | 68.33 | 1 | LOXIMVI-signal3 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2817.60 | p-Rb(S807/S811) | 205.31 | p-cJun(S73) | 68.42 | 2 | LOXIMVI-signal3 | E7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2617.50 | p-Rb(S807/S811) | 180.39 | p-cJun(S73) | 69.18 | 2 | LOXIMVI-signal3 | G8 |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 688.19 | p-Rb(S807/S811) | 44.09 | p-cJun(S73) | 69.41 | 2 | WM115-signal3 | C3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 609.38 | p-Rb(S807/S811) | 99.97 | p-ERK(T202/Y204) | 70.02 | 2 | LOXIMVI-signal1 | G2 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 680.51 | p-Rb(S807/S811) | 40.34 | p-cJun(S73) | 71.07 | 2 | Colo858-signal3 | C4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 356.71 | p-Rb(S807/S811) | 166.33 | p-ERK(T202/Y204) | 71.63 | 1 | LOXIMVI-signal1 | F6 |